Hams F-12, with L-Glutamine

Hams F-12, with L-Glutamine Specification

Storage Instructions
+2 DegreeC to +8 DegreeC

CAS No
091242354

Physical State
Liquid

Packaging Type
Bottle

Size
500 mL

About Hams F-12, with L-Glutamine

Ham's F-12 Medium (Ham's F-12 Nutrient Mix) is a widely used basal medium for cell culture. It was developed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Hams F-12 is the medium of choice for a Clonal Toxicity Assay (CTA). This formulation of Ham's F-12 medium is supplemented with L-Glutamine.

Key Features and Benefits

Hams F-12 with L-Glutamine is formulated to support the growth and maintenance of diverse cell lines. Its liquid form ensures easy handling and precise measurement, while the presence of L-Glutamine promotes enhanced cell metabolism. Packaged securely in a 500 mL bottle, it offers stability and convenience for laboratory storage and usage in India.

Proper Storage and Handling

The medium should be stored at temperatures between +2C to +8C to preserve its composition and activity. This temperature range prevents degradation of sensitive components, ensuring maximum performance throughout its shelf life.

FAQ's of Hams F-12, with L-Glutamine:

Q: How should Hams F-12 with L-Glutamine be stored?

A: It is important to store Hams F-12 with L-Glutamine at a temperature range of +2C to +8C to maintain its efficacy and prevent the breakdown of nutrients, particularly L-Glutamine.

Q: What is the process for using Hams F-12 in cell culture?

A: To use Hams F-12 with L-Glutamine, simply dispense the required volume from the 500 mL bottle into sterile culture vessels under aseptic conditions. It serves as a culture medium suitable for supporting the proliferation of a variety of cell lines.

Q: When is Hams F-12 with L-Glutamine typically used?

A: This medium is commonly used during the establishment, maintenance, and sub-culturing of mammalian cells in laboratory research, especially when enhanced cell growth and metabolism are required.

Q: Where is Hams F-12 with L-Glutamine manufactured and supplied?

A: Hams F-12 with L-Glutamine is manufactured, supplied, and distributed by reputable entities in India, ensuring accessibility and compliance with regional quality standards.

Q: What are the advantages of using Hams F-12 with L-Glutamine?

A: The primary benefit is its reliable support for robust cell growth, thanks to L-Glutamine. The liquid format ensures convenient handling, and its tailored formulation offers consistent performance in laboratory settings.

Q: How does L-Glutamine contribute to cell culture?

A: L-Glutamine is a key nutrient that fuels energy production and biosynthesis in cells, making Hams F-12 particularly effective for cultures that demand elevated metabolic activity.

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