About MEM with Earles Salts, with Stable Glutamine
MEM (Minimum Essential Medium) with Earle's Salts was originally developed by Harry Eagle and from there modified in several ways (e.g. alpha modification) to suit many cell culture applications. It is used for cultivation of numerous suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This modification of MEM with Earle's Salts is supplemented with Stable Glutamine. Stable glutamine (also known as L-Alanyl-L-Glutamine) is a direct substitute for L-glutamine and has an increased stability.
Enhanced Cell Growth SupportMEM with Earles Salts and stable glutamine delivers vital nutrients and amino acids, supporting robust growth and maintenance of diverse mammalian cell lines in research and laboratory environments.
Optimized Stability and Shelf LifeFormulated with stable glutamine, this medium minimizes degradation over time, maintaining consistent performance and reducing wastage during storage at recommended temperatures (+2C to +8C).
Convenient Packaging and HandlingWith a 500 mL bottle packaging, the liquid medium is simple to dispense and compatible with standard laboratory workflows, ensuring ease of use from leading Indian suppliers, manufacturers, and traders.
FAQ's of MEM with Earles Salts, with Stable Glutamine:
Q: How should MEM with Earles Salts and stable glutamine be stored?
A: The product should be stored at temperatures between +2C and +8C to preserve its stability and ensure optimal performance during use.
Q: What are the main benefits of using MEM with Earles Salts and stable glutamine in cell culture?
A: This medium provides essential nutrients for cell growth and, with its stable glutamine formulation, offers extended shelf life and reduced degradation, supporting consistent experimental results.
Q: When is it appropriate to use this medium in the laboratory?
A: MEM with Earles Salts is ideal for culturing a broad spectrum of mammalian cells, whether for routine maintenance, expansion, or experimentation in research settings.
Q: Where is MEM with Earles Salts with stable glutamine manufactured and supplied?
A: This medium is manufactured, supplied, and traded in India, catering to local and international standards for research quality.
Q: What is the recommended process for using this liquid medium?
A: Prior to use, ensure the medium is brought to room temperature under sterile conditions. Dispense the required amount into cell culture vessels according to your protocol, following standard aseptic techniques.
Q: How does the inclusion of stable glutamine improve this formulation?
A: Stable glutamine resists breakdown during storage, ensuring the medium retains its nutrient content, leading to more reliable cell growth and fewer changes in experimental conditions over time.