Phenylmethylsulfonyl Fluoride (PMSF), 1 Gm

Phenylmethylsulfonyl Fluoride (PMSF), 1 Gm Specification

Molecular Formula
C7H7FO2S

Physical State
Powder

CAS No
329-98-6

Color
White

Packing condition
Light-protected

Flash Point
137°C

Stability
Sensitive to moisture

Solubility
Ethanol, DMSO

Transport
Regulated chemical

Hazard Class
Toxic, irritant

Form
Solid

Reactivity
Hydrolyzes in water

Odor
Odorless

About Phenylmethylsulfonyl Fluoride (PMSF), 1 Gm

Phenylmethylsulfonyl fluoride ,inhibits serine proteases such as trypsin and chymotrypsin. Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase

Application in Biochemical Research

PMSF is primarily used in enzyme inhibition studies, particularly to block serine proteases in biological samples. Researchers depend on its effectiveness to protect protein integrity during sample processing. Its rapid action and specificity are valued in complex assay systems. Always handle with care, using gloves and working in a dry, protected environment.

Handling and Storage Guidelines

Due to its moisture sensitivity and toxic nature, PMSF should be stored in tightly sealed containers, protected from light and humidity. Use only in well-ventilated areas, ideally inside a fume hood, to minimize exposure. Dispose of residuals according to hazardous chemical regulations and always keep it away from water-based solutions during storage.

FAQ's of Phenylmethylsulfonyl Fluoride (PMSF), 1 Gm:

Q: How should Phenylmethylsulfonyl Fluoride (PMSF) be handled and stored?

A: PMSF should be handled in a fume hood with personal protective equipment due to its toxic and irritant properties. Store it in an airtight, light-protected container in a cool, dry place, avoiding any contact with moisture or water to maintain stability.

Q: What is the main use of PMSF in laboratory settings?

A: PMSF is widely used as a serine protease inhibitor in biochemical and molecular biology research, preventing protein degradation during extraction and sample preparation.

Q: When does PMSF lose its effectiveness?

A: PMSF rapidly hydrolyzes and becomes inactive when exposed to water or moisture, so it should be dissolved in ethanol or DMSO and used immediately in experimental protocols.

Q: Where can I purchase PMSF in India?

A: PMSF can be sourced from various manufacturers, suppliers, and traders across India. Ensure you select a reputable chemical supplier who provides products with appropriate regulatory certifications.

Q: What is the process for dissolving PMSF for use in experiments?

A: Dissolve PMSF in ethanol or DMSO, as specified by your protocol, to prepare a stock solution. Use freshly prepared solutions and add them to samples immediately, minimizing exposure to moisture.

Q: Why is PMSF transported as a regulated chemical?

A: Due to its toxic, irritant nature and specific storage requirements, PMSF is classified as a regulated chemical. Transportation must comply with hazardous material guidelines to ensure safety.

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