RPMI 1640, w/o L-Glutamine, w/o Phenol Red

RPMI 1640, w/o L-Glutamine, w/o Phenol Red Specification

Size
500 mL

Storage Instructions
+2 DegreeC to +8 DegreeC

Shelf Life
1 Years

Purity
High

Physical State
Liquid

CAS No
091261654

RPMI 1640, w/o L-Glutamine, w/o Phenol Red Trade Information

Minimum Order Quantity
1 Kilograms

Delivery Time
5-10 Days

About RPMI 1640, w/o L-Glutamine, w/o Phenol Red

RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 comes without L-Glutamine and without Phenol Red. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.

Specialized Cell Culture Medium

This RPMI 1640 variant is specifically formulated without L-Glutamine and Phenol Red, making it ideal for sensitive cell cultures and specialized assays. By excluding these components, researchers can customize supplementation to individual experimental requirements, ensuring maximum flexibility and performance for advanced cell biology studies.

Safe and Reliable Storage

Store RPMI 1640 at +2C to +8C to preserve its efficacy and extend shelf life. Proper storage is critical to prevent contamination and maintain the integrity of nutrients and buffer systems within the medium. Its one-year shelf life offers convenience for routine and long-term research needs.

FAQ's of RPMI 1640, w/o L-Glutamine, w/o Phenol Red:

Q: How should RPMI 1640 without L-Glutamine and Phenol Red be stored to ensure longevity?

A: This medium must be kept refrigerated at a temperature range between +2C and +8C. Maintaining this storage condition helps preserve its chemical stability and ensures it remains effective throughout its one-year shelf life.

Q: What are the advantages of using RPMI 1640 without L-Glutamine and Phenol Red?

A: Omitting L-glutamine allows for customized supplementation, which is ideal for experiments where glutamine stability is critical. The absence of phenol red benefits sensitive applications, such as hormone or fluorescence-based assays, by eliminating unnecessary background interference.

Q: When is this RPMI 1640 formulation most beneficial in the cell culture process?

A: This formulation is most useful when working with cell types sensitive to phenol red or in applications requiring precise control over glutamine concentrations, such as cytotoxicity testing or gene expression analysis.

Q: Where is this RPMI 1640 medium available for purchase?

A: RPMI 1640 without L-Glutamine and Phenol Red is supplied by manufacturers, suppliers, and traders based in India, typically in 500 mL liquid bottles, supporting both academic and industrial research sectors.

Q: What processes require adding L-glutamine separately to this medium?

A: L-glutamine must be added separately whenever your experimental setup or cell line needs this amino acid for optimal growth. This allows researchers to tailor the amount and freshness of glutamine in each experiment.

Q: How can I use this medium for cell culture work?

A: Simply supplement the medium with required additives (such as L-glutamine and antibiotics if needed), warm it to incubation temperature, and use as per standard cell culture protocols for seeding, maintenance, or experimental treatments.

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