RPMI 1640, w/o L-Glutamine, with 25 mM HEPES

RPMI 1640, w/o L-Glutamine, with 25 mM HEPES Specification

Physical State
Liquid

Size
500 mL

Storage Instructions
+2 DegreeC to +8 DegreeC

Packaging Type
Bottle

CAS No
091261354

About RPMI 1640, w/o L-Glutamine, with 25 mM HEPES

RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 comes without L-Glutamine and is supplemented with 25 mM HEPES. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.

Optimized for Cell Culture Applications

RPMI 1640 without L-Glutamine, with 25 mM HEPES, provides a balanced environment suited for a variety of mammalian cells. HEPES buffer enhances pH stability, making it suitable for experiments requiring controlled conditions.

Reliable Packaging and Safety

Offered in a secure 500 mL bottle, this medium ensures convenient handling and reduces contamination risks. Liquid formulation means it's ready to use without additional preparation steps, streamlining cell culture workflows.

Storage and Shelf Life Considerations

For optimal stability and performance, store the product at temperatures between +2C and +8C. Proper storage conditions help maintain the medium's quality and ensure reproducible results throughout its shelf life.

FAQ's of RPMI 1640, w/o L-Glutamine, with 25 mM HEPES:

Q: How should RPMI 1640, without L-Glutamine and with 25 mM HEPES, be stored?

A: It should be stored at temperatures between +2C and +8C to maintain stability and efficacy. Always keep the bottle tightly closed and avoid repeated freeze-thaw cycles.

Q: What are the primary uses of this RPMI 1640 formulation?

A: This formulation is used for culturing mammalian cells, especially for experiments where controlled addition of L-Glutamine is required. The presence of HEPES buffer provides enhanced pH stability during cell culture.

Q: When is it necessary to choose a medium without L-Glutamine?

A: A glutamine-free medium is chosen when researchers need to add glutamine freshly or omit it for particular studies. This flexibility helps prevent glutamine degradation and supports specialized experimental designs.

Q: Where is this RPMI 1640 medium sourced and supplied in India?

A: This medium is available from manufacturers, suppliers, and traders based in India, ensuring prompt local availability and support for research laboratories nationwide.

Q: What is the process for using this product in cell culture?

A: Simply pour the desired amount directly from the bottle into your culture vessels. Supplement with L-Glutamine or other additives as required by your protocol before introducing cells.

Q: What benefits does the inclusion of HEPES buffer provide in this formulation?

A: HEPES buffer significantly improves the medium's ability to maintain a stable pH, even in open incubator conditions, which is crucial for sensitive cell lines and consistent experimental outcomes.

Tell us about your requirement