RPMI 1640, with L-Glutamine, with 25 mM HEPES

RPMI 1640, with L-Glutamine, with 25 mM HEPES Specification

Purity
High

Packaging Type
Bottle

Size
500ml

Physical State
Liquid

Shelf Life
1 Years

CAS No
091261254

Type
Industrial Lab Chemicals

RPMI 1640, with L-Glutamine, with 25 mM HEPES Trade Information

Minimum Order Quantity
1 Piece

Supply Ability
1000 Pieces Per Month

Delivery Time
7 Days

About RPMI 1640, with L-Glutamine, with 25 mM HEPES

RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 is supplemented with L-Glutamine and 25 mM HEPES. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.

Superior Formulation for Reliable Results

RPMI 1640, enriched with L-Glutamine and buffered with 25 mM HEPES, ensures a stable and physiologically relevant environment for cell cultures. Its liquid form and high purity standards provide excellent support for a wide range of industrial and research applications, helping scientists achieve reproducible outcomes.

Optimized Packaging for Laboratory Efficiency

Packaged in 500ml bottles, this medium is designed to minimize contamination and maintain quality during storage and usage. The bottle packaging allows for convenient handling in busy laboratory settings, making everyday procedures more efficient.

FAQ's of RPMI 1640, with L-Glutamine, with 25 mM HEPES:

Q: How should RPMI 1640 with L-Glutamine and HEPES be stored to maintain its purity?

A: It should be stored at 2-8C in its original bottle, away from direct sunlight and contamination sources, to preserve its high purity and maintain performance throughout its 1-year shelf life.

Q: What benefits does the addition of 25 mM HEPES provide to this medium?

A: The inclusion of 25 mM HEPES acts as a buffer, enhancing pH stability during cell culture processes, particularly in environments where CO2 control is limited, thus ensuring optimal cell growth and viability.

Q: When is it recommended to use RPMI 1640 with L-Glutamine for cell culture?

A: This medium is ideal for use in cell culture applications requiring consistent growth and metabolism, such as immunological, hematological, or hybridoma cell studies in industrial laboratory research.

Q: Where is this product available and who supplies it?

A: RPMI 1640 with L-Glutamine and HEPES is manufactured, supplied, and traded in India and can be sourced directly from laboratories and industrial chemical suppliers in the region.

Q: What is the recommended process for preparing this medium for use in laboratory experiments?

A: Before use, the medium should be equilibrated to room temperature and, if desired, supplemented with recommended additives. Aseptic techniques are essential during handling to prevent contamination.