About RPMI 1640, with Stable Glutamine
RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 is supplemented with Stable Glutamine. Stable glutamine (also known as L-Alanyl-L-Glutamine) is a direct substitute for L-glutamine and has an increased stability. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.
Stable Glutamine for Extended StabilityRPMI 1640 features a stabilized form of glutamine, minimizing degradation and ensuring better shelf life compared to traditional L-glutamine. This leads to improved cell performance and greater reproducibility in your experiments.
Convenient Liquid FormatSupplied as a ready-to-use liquid in a 500 mL bottle, the medium eliminates the need for reconstitution, making laboratory workflows more efficient and reducing the risk of error.
Adhering to Quality StandardsProduced in India by reputable manufacturers and distributors, this RPMI 1640 formulation meets rigorous quality controls. Its packaging maintains sterility and integrity during storage and transportation.
FAQ's of RPMI 1640, with Stable Glutamine:
Q: How should I store RPMI 1640 with Stable Glutamine to ensure maximum shelf life?
A: Store the bottle at a temperature between +2C and +8C as recommended to preserve the stability of the glutamine and other nutrients.
Q: What is the primary benefit of using RPMI 1640 with Stable Glutamine over traditional formulations?
A: The inclusion of stable glutamine prolongs the medium's effectiveness, reduces ammonia buildup, and enhances cell viability during extended culture periods.
Q: When should RPMI 1640 with Stable Glutamine be used in cell culture work?
A: This medium is ideal for both routine and specialized mammalian cell culture applications, particularly when extended culture periods or high viability are needed.
Q: Where is RPMI 1640 with Stable Glutamine produced and supplied from?
A: RPMI 1640 with Stable Glutamine is manufactured, supplied, and traded by companies in India, ensuring local availability and support.
Q: What is the process for using RPMI 1640 with Stable Glutamine in my experiments?
A: Simply aseptically transfer the required volume from the 500 mL bottle to your culture vessel. No additional supplementation or reconstitution is necessary.
Q: How does the physical state and packaging of this product benefit the end-user?
A: Its liquid state in a sterile, resealable 500 mL bottle offers convenience, reduces prep time, and minimizes contamination risks compared to powdered media.