SPINeasy DNA/RNA/Protein All In One Kit

SPINeasy DNA/RNA/Protein All In One Kit Specification

Binding Capacity
50 g DNA

Yield Quality
High purity

Manuals Included
Yes

Protocol Time
<60 minutes

Format
Spin column

Elution Volume
2050 l

Sample Capacity
Up to 50 mg

RNase Free
Yes

Protein Safe
Yes

About SPINeasy DNA/RNA/Protein All In One Kit

SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica-membrane spin-columns to isolate DNA, RNA and protein components from the same sample, without the use of toxic substances such as phenol and chloroform. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep Instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA and proteins are then sequentially purified from the same lysate. Briefly, DNA is adsorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed and resuspended. Each molecular component is then immediately available for their respective downstream applications.

Effortless Multi-molecule Extraction

This versatile kit enables researchers to extract DNA, RNA, and protein concurrently from a single sample, saving both time and resources. The advanced spin column method ensures high-quality results and reliable reproducibility. Whether processing tissue, cell pellets, or cultured cells, the protocol accommodates up to 50 mg of input material per run, supporting diverse workflows and applications in molecular biology.

Fast and Reliable Workflow

With a protocol time of less than 60 minutes, the SPINeasy All In One Kit is engineered for rapid processing without compromising performance. The RNase-free and protein-safe design guarantees the integrity of extracted biomolecules, making the kit ideal for downstream applications in genomics, transcriptomics, and proteomics research.

FAQ's of SPINeasy DNA/RNA/Protein All In One Kit:

Q: How do I use the SPINeasy DNA/RNA/Protein All In One Kit for sample extraction?

A: Simply follow the clearly outlined steps in the included manuals: add your sample (up to 50 mg) to the lysis buffer, bind the nucleic acids and proteins to the spin column, perform washing steps, and finally elute the purified DNA, RNA, or protein in 20-50 l.

Q: What types of samples can be processed with this kit?

A: This kit is suitable for a range of biological specimens, including tissue, cultured cells, and cell pellets, accommodating up to 50 mg of starting material per preparation with high yield and purity.

Q: When should I choose the SPINeasy All In One Kit over separate extraction kits?

A: Opt for this kit when you require efficient, concurrent extraction of DNA, RNA, and protein from a single sample source for streamlined workflows and minimized sample consumption.

Q: Where can the SPINeasy Kit be sourced in India?

A: The kit is available from local manufacturers, suppliers, and authorized traders across India. Contact your scientific supplier or visit distributor websites for purchasing options.

Q: What benefits does the spin column format provide in this kit?

A: The spin column system ensures rapid processing, minimizes sample loss, and consistently delivers high purity nucleic acids and proteins, optimizing results for downstream molecular applications.

Q: Is the protocol safe for protein and RNA applications?

A: Yes, the protocol is completely RNase-free and protein safe, ensuring both nucleic acids and proteins remain intact and suitable for sensitive analyses.

Q: What is included with the SPINeasy Kit upon purchase?

A: Every kit comes with a comprehensive user manual, all necessary buffers, spin columns, and reagents required for efficient DNA, RNA, and protein extraction.

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