1X RPMI 1640 Medium

1X RPMI 1640 Medium Specification

Ph Level
6.9 - 7.4

CAS No
091260354

Size
100 mL & 500 mL

Packaging Type
Bottle

Type
Other

About 1X RPMI 1640 Medium

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L, 1000 mg/L sodium bicarbonate instead of 2000 mg/L, and 20 mM HEPES.

Reliable Cell Culture Performance

The 1X RPMI 1640 Medium offers consistency and stability required for vital biological experiments. Designed to match physiological conditions, it enables robust growth and maintenance of lymphocytes and cancer cell lines, supporting accurate and reproducible research outcomes.

Convenient Packaging Options

This medium is available in both 100 mL and 500 mL bottle formats, making it suitable for varied laboratory scales. Each bottle is manufactured and supplied by trusted sources in India, ensuring dependable quality every time it reaches your workspace.

FAQ's of 1X RPMI 1640 Medium:

Q: How should I use 1X RPMI 1640 Medium in my cell culture experiments?

A: To use 1X RPMI 1640 Medium, simply warm the medium to 37C and directly add it to your culture vessels. It is ready-to-use and does not require further dilution, providing an efficient workflow for cell maintenance and propagation.

Q: What types of cells are best supported by RPMI 1640 Medium?

A: RPMI 1640 Medium is commonly used for cultivating mammalian cells, especially lymphocytes and various cancer cell lines. Its balanced formulation supports optimal cell growth and viability in research and diagnostic settings.

Q: When should I choose the 100 mL versus 500 mL packaging size?

A: Opt for the 100 mL bottle for small-scale or pilot studies and use the 500 mL option for routine, larger-scale applications. Both packaging types offer the same quality medium, allowing you to select based on your experimental requirements.

Q: Where is this RPMI 1640 Medium manufactured and supplied from?

A: This product is manufactured, supplied, and traded by reputable sources within India. Local sourcing ensures a reliable supply chain and product integrity.

Q: What is the recommended storage process for RPMI 1640 Medium?

A: Store the medium in a refrigerator at 2-8C, away from direct sunlight. Ensure the cap is tightly closed after each use to maintain sterility and effectiveness throughout its shelf life.

Q: What are the key benefits of using this RPMI 1640 Medium?

A: The main benefits include a stable pH range (6.9-7.4), easy-to-use bottle packaging, and compatibility with a range of mammalian cells. Its proven formula supports reproducible results in both basic and advanced cell culture research.

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