SPINeasy DNA Kit for Plant

SPINeasy DNA Kit for Plant Specification

CAS No
116535050

RNase Included
Yes

Compatibility
PCR, qPCR ready

Sample Type
Plant tissue

Protocol
Manual

Elution Volume
50100 l

Yield
Up to 30 g DNA

Format
Spin column based

Column Capacity
600 l

Isolation Time
~30 minutes

About SPINeasy DNA Kit for Plant

SPINeasy DNA Kit for Plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed. High quality gDNA can be extracted from polysaccharide and polyphenolic-rich plants. The use of our specially formulated Lysis Buffer PD and Lysing Matrix A in combination with FastPrep Instruments from MP Biomedicals enables highly efficient lysis of various types of samples within seconds. For DNA purification, Column PD and kit buffers are designed to deliver gDNA extracts of high yield and purity which are compatible with downstream molecular biology applications such as PCR, restriction digestion and sequencing.

Efficient DNA Extraction from Plant Tissue

Designed especially for plant samples, the SPINeasy DNA Kit combines speed, simplicity, and reliable results. It accommodates a broad range of plant tissues and delivers high-quality DNA suitable for PCR and qPCR within 30 minutes. The spin column-based protocol streamlines the purification process without the need for complicated equipment.

Optimized Protocol with User-Friendly Features

This kit offers a manual yet straightforward protocol suitable for most standard laboratories. With an elution volume adjustable between 50-100 l and a column capacity of 600 l, users can process varying sample sizes while maximizing DNA yields. The inclusion of RNase ensures preparations are virtually free of RNA contaminants.

FAQ's of SPINeasy DNA Kit for Plant:

Q: How does the SPINeasy DNA Kit for Plant isolate DNA from plant tissue?

A: The kit uses a spin column-based method that binds DNA while contaminants are washed away. After lysis and purification, DNA is eluted in 50-100 l, resulting in high purity suitable for downstream applications.

Q: What sample types are compatible with this kit?

A: The SPINeasy DNA Kit is specifically developed for a broad variety of plant tissues, including leaves, stems, and roots, allowing flexible applications in plant genomics research.

Q: When should I use the SPINeasy DNA Kit for Plant?

A: Use this kit whenever you need to rapidly isolate high-quality DNA from plant tissue for applications such as PCR or qPCR. Its quick 30-minute protocol is ideal for routine and high-throughput workflows.

Q: Where is the SPINeasy DNA Kit for Plant available?

A: The kit is manufactured, supplied, and distributed by authorized traders across India. Researchers and labs within India can source it easily from certified vendors.

Q: What is the DNA yield and purity achieved using this kit?

A: You can expect DNA yields of up to 30 g, with purity suitable for sensitive techniques like PCR and qPCR, thanks to efficient binding and washing steps in the spin column protocol.

Q: How is RNA contamination minimized during extraction?

A: RNase is included in the kit to degrade RNA during the extraction process, ensuring that your resulting DNA preparation is free from RNA contamination.

Q: What are the benefits of using a manual, spin column-based DNA isolation kit?

A: Manual, spin column-based protocols are straightforward, require basic lab equipment, and offer fast, reproducible results with high purity DNA. This makes the kit ideal for routine lab use where time and consistency are essential.

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