DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES

DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES Specification

Size
500 mL

Storage Instructions
+2 DegreeC to +8 DegreeC

CAS No
091247954

Packaging Type
Bottle

About DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES

DMEM High Glucose (Dulbecco's Modified Eagle Medium) is a standard liquid growth medium that provides basic nutrients for all cell culture applications. This formulation of DMEM comes with stable glutamine and 25 mM HEPES. Stable glutamine (also known as L-Alanyl-L-Glutamine) is a direct substitute for L-glutamine and has an increased stability.

Enhanced Nutrient Support for Cell Cultures

DMEM High Glucose (4.5 g/l) is engineered with stable glutamine and buffered with 25 mM HEPES, delivering consistent nutrient supply and optimal pH stability. The added glucose and glutamine support energetic and biosynthetic demands of rapidly proliferating cells. The medium is suitable for a broad array of mammalian cell lines, making it a preferred choice for researchers.

Reliable Storage and Packaging

Each 500 mL bottle is securely packaged to preserve the stability and sterility of the medium until use. To maintain its efficacy, the medium should be stored between +2C and +8C and protected from prolonged light exposure. These storage guidelines ensure the medium's quality is uncompromised during its shelf life.

FAQ's of DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES:

Q: How should DMEM High Glucose (4.5 g/l) with stable glutamine and 25 mM HEPES be stored?

A: To preserve quality and effectiveness, DMEM High Glucose should be stored at temperatures between +2C and +8C. Ensure the bottle is tightly closed and avoid repeated freeze-thaw cycles or prolonged exposure to light.

Q: What are the primary benefits of using this DMEM formulation in cell culture?

A: This DMEM offers high glucose concentration for enhanced cellular energy, stable glutamine for biosynthesis, and HEPES buffering for optimal pH maintenance. Together, these features provide a robust environment for consistent and vigorous mammalian cell growth.

Q: When should I choose DMEM High Glucose with stable glutamine and HEPES over standard DMEM?

A: Select this formulation when working with cell lines that demand elevated energy or experience acidic conditions. The combination of high glucose and HEPES buffer ensures better resilience during high-density cultures or stressful experimental setups.

Q: Where is this DMEM variant commonly utilized?

A: This medium is used in leading research and industrial laboratories throughout India, particularly in mammalian cell research, biotechnology, pharmaceutical development, and academic environments.

Q: What is the recommended usage process for the 500 mL DMEM High Glucose bottle?

A: Before opening, bring the bottle to room temperature. Sterilize the exterior, and aseptically transfer the needed volume into cell culture vessels. Always work under aseptic conditions to prevent contamination.

Q: How does the inclusion of stable glutamine and HEPES buffer improve the cell culture process?

A: Stable glutamine reduces ammonia buildup and increases media shelf life, while 25 mM HEPES maintains stable pH during culture, especially outside CO2 incubators, promoting healthier and more consistent cell growth outcomes.

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