Zymolyase 100T, (100KU/g)

Zymolyase 100T, (100KU/g) Specification

Usage
Yeast Cell Culture | Used to lyse cell wall of yeast

Physical State
Liquid

CAS No
08320932

Storage Instructions
Store at -20°C

Application
Yeast cell wall lysis

Appearance
Pale yellow powder

Molecular Weight
~20 kDa

Purity(%)
>90%

Packaging Type
Vial

Purity
>90%

Density
Not determined

Ph Level
7.5 ± 0.5

Activity
100 KU/g

Grade
Research grade

Type
Enzyme preparation

Formulation
Lyophilized powder

Shelf Life
24 months

Size
100KU/g

About Zymolyase 100T, (100KU/g)

Zymolyase-100T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells in -1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the -1,3-glucan linkages releasing laminaripentaose as the principal product.

Optimized Yeast Cell Wall Lysis

Zymolyase 100T is engineered to efficiently break down yeast cell walls, facilitating the quick release of intracellular contents such as nucleic acids and proteins. Its robust activity and high purity make it highly suitable for laboratory protocols involving yeast cell research, ensuring reproducible and fast results every time.

Superior Stability and Convenient Handling

This enzyme comes as a stable, lyophilized powder packaged securely in vials, ensuring its activity is preserved throughout its 24-month shelf life. With recommended storage at -20C, Zymolyase 100T remains ready for immediate use, reducing preparation time for your laboratory workflows.

FAQ's of Zymolyase 100T, (100KU/g):

Q: How should Zymolyase 100T be stored to maintain its activity?

A: Zymolyase 100T should be stored at -20C in its original vial to preserve its enzymatic activity and extend its shelf life up to 24 months. Avoid repeated freeze-thaw cycles for best results.

Q: What is the main application of Zymolyase 100T in the laboratory?

A: The primary application of Zymolyase 100T is the lysis of yeast cell walls. It is commonly used to release intracellular components, such as DNA, RNA, or proteins, for downstream analysis in yeast cell culture experiments.

Q: When is it beneficial to use Zymolyase 100T in research protocols?

A: Zymolyase 100T is most beneficial when you need to efficiently disrupt the yeast cell wall to extract cellular material, streamline sample preparation, or improve yields in molecular biology and biochemical analyses involving yeast.

Q: Where is Zymolyase 100T typically used?

A: This enzyme preparation is widely used in research laboratories, biotechnology centers, and academic institutions that focus on yeast genetics, molecular biology, and biochemical studies.

Q: What is the recommended process for using Zymolyase 100T for yeast cell lysis?

A: Suspend the lyophilized powder in an appropriate buffer maintaining a pH of around 7.5, add it to the yeast cell suspension, and incubate under optimal conditions until lysis is achieved. Follow protocol specifics for your intended downstream workflow.

Q: What benefits does Zymolyase 100T offer over other lysis methods?

A: Zymolyase 100T provides a high-purity, consistent enzymatic solution, enabling gentle yet efficient lysis of yeast cell walls. It ensures minimal damage to intracellular contents, thus improving the quality and yield of extracted materials.

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