Sodium Chloride, Molecular Biology Reagent

Sodium Chloride, Molecular Biology Reagent Specification

Melting Point
1474 Degree F

Usage
Buffer Component | Precipitation of DNA | Chemical sequencing of DNA | Column Chromatography

Physical State
Powder

CAS No
0219484801

Grade
Molecular Biology

Packaging Type
HDPE bottle

Boiling point
2669 °F

Molecular Weight
58.44 g/mol

Type
Reagent Grade

Appearance
White crystalline

Ph Level
7 (neutral)

Size
500 g, 1 kg

Shelf Life
5 years

Purity
>=99.5%

Purity(%)
>=99.5%

Molecular Formula
NaCl

Application
Molecular biology research

Density
2.16 g/cm³

Storage Instructions
Store at room temperature

About Sodium Chloride, Molecular Biology Reagent

Sodium chloride is used in a wide variety of biochemical applications, including intravenous fluids (normal saline is 0.9% w/v in water10), density gradients3,6, a diluent to increase ionic strength in buffers or culture media and in salt-out procedures in the isolation of DNA.8 It has also been used in high concentrations for preservation of foods since bacteria cannot grow in high salt conditions. A salt-ice mixture in the ratio of approximately 33 g sodium chloride to 100 g ice (at -1C) will drop in temperature to as low as -21C, depending on the rate of stirring and the size of the ice chunks.

High Purity for Accurate Results

This sodium chloride reagent is produced with a purity of at least 99.5%, making it ideal for molecular biology research where impurities may compromise experimental outcomes. Its neutral pH and stable crystalline form ensure optimal compatibility across a range of sensitive protocols.

Versatile Applications in Molecular Biology

From acting as a critical buffer component and aiding in DNA precipitation to supporting chemical sequencing and chromatography, this reagent serves multiple essential functions in the lab. Its consistent quality enhances both reproducibility and reliability in experimental processes.

Secure and Convenient Packaging

Packaged in HDPE bottles, the sodium chloride is protected from moisture and contaminants. Available in multiple sizes (500 g and 1 kg), this reagent is easy to handle, store, and dispense, meeting both small- and large-scale laboratory demands.

FAQ's of Sodium Chloride, Molecular Biology Reagent:

Q: How should Sodium Chloride, Molecular Biology Reagent be stored to maintain its purity?

A: Store the reagent at room temperature in its original, tightly-sealed HDPE bottle to protect it from moisture and contamination, ensuring a stable shelf life of up to five years.

Q: What are the typical laboratory uses for this Sodium Chloride Molecular Biology Reagent?

A: This sodium chloride is primarily used as a buffer component, for precipitation of DNA, chemical sequencing of DNA, and in column chromatography protocols within molecular biology research.

Q: When is it recommended to use this high-purity sodium chloride in DNA purification protocols?

A: It is best used during DNA precipitation steps or when preparing solutions that require stringent purity to avoid introducing contaminants that could affect the downstream analysis.

Q: Where is the sodium chloride sourced and manufactured?

A: This product is manufactured, supplied, and traded in India, adhering to strict molecular biology reagent grade standards.

Q: What is the process for preparing solutions using this sodium chloride reagent?

A: Accurately weigh the desired amount of sodium chloride, add to distilled water, and dissolve completely. Ensure that all glassware and equipment are clean to prevent cross-contamination, and use freshly prepared solutions when possible.

Q: How does using this molecular biology grade sodium chloride benefit experimental reproducibility?

A: The high purity level (99.5%) and neutral pH minimize variables that could interfere with physiological or biochemical processes, supporting precise and repeatable experimental outcomes.

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