About Iscove's Modified Dulbecco's Medium, with L-Glutamine, without Supplements
Iscove's Modified Dulbecco's Medium (IMDM) is a complex and enriched growth medium for cell culture. It is a modification of Dulbecco's Modified Eagle Medium (DMEM) and contains selenium as well as additional amino acids and vitamins. IMDM medium is usually supplemented with Fetal Bovine Serum (FBS) and is used for the cultivation of specialized cell types, such as Jurkat, COS-7, and macrophage cells. This formulation of IMDM comes with L-Glutamine.
Optimized for Flexibility in Cell CultureIMDM with L-Glutamine but without additional supplements is tailored for applications requiring precise control over culture conditions. This formulation allows users to add supplements as needed for specific cell lines or experiments, providing unmatched versatility in industrial settings and research laboratories.
Strict Quality and Purity AssuranceManufactured to a 99% purity specification, this medium assures reliable performance and reproducibility. Rigid quality checks at every stage ensure that each batch meets the highest industrial standards, making it a trusted choice for consistent cell growth and viability.
Simple Storage and HandlingFor optimal stability and usability, the medium should be stored at temperatures between 2 to 8C. With a shelf life of 12 months, users benefit from extended usability without concerns about product degradation, ensuring a dependable supply for ongoing industrial applications.
FAQ's of Iscove's Modified Dulbecco's Medium, with L-Glutamine, without Supplements:
Q: How should Iscove's Modified Dulbecco's Medium be stored to maintain its quality?
A: To preserve its quality and efficacy, the medium should be stored at a temperature between 2 to 8C. Ensure that the container is tightly sealed and protected from direct light during storage to maximize its 12-month shelf life.
Q: What makes this IMDM formulation suitable for industrial use?
A: This IMDM is formulated with L-Glutamine and without added supplements, allowing customizable preparation tailored for diverse industrial cell culture processes. Its high purity (99%) and liquid state support consistent results in large-scale applications.
Q: When is supplementation necessary with this medium?
A: Supplements should be added when your specific cell line or experimental protocol requires additional nutrients or growth factors not present in the base medium. This allows tailored enrichment based on experimental objectives.
Q: Where is this IMDM variant manufactured and available?
A: This product is manufactured, supplied, and traded in India, ensuring availability and support for industrial customers across the region and potentially internationally through established distributors.
Q: What is the recommended process for using this medium in cell culture?
A: Thaw the liquid medium at room temperature if refrigerated, then aseptically add any required supplements or antibiotics. Afterwards, use the prepared medium according to the needs of your cell culture protocol for optimal growth and maintenance.
Q: What are the benefits of using IMDM with L-Glutamine but without supplements?
A: The absence of supplements offers flexibility, so you can specifically tailor the medium to your experimental needs, reducing interference from unnecessary additives and promoting optimal cell growth for your intended application.