Hygromycin B, Phytopure, 1gm

Hygromycin B, Phytopure, 1gm Specification

Physical State
powder

Usage
In gene transfer experiments as a selection agent.

CAS No
31282-04-9

Shelf Life
24 months (unopened)

Endotoxin Level
Low

Form
Solid

Sterility
Non-sterile

Color
Off-white

Target Organism
Bacteria, plants

Solubility
Water soluble

About Hygromycin B, Phytopure, 1gm

An aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.

High Purity and Versatility

With its water solubility and off-white, powdery form, Hygromycin B, Phytopure is optimized for ease of preparation and use in various applications. Its low endotoxin level ensures minimal interference with experimental outcomes, supporting both bacterial and plant systems.

Reliable Selection Agent in Gene Transfer

One of the most vital uses of Hygromycin B is in genetic engineering, serving as a robust selection agent. Its efficacy in screening genetically modified cells guarantees precise results in gene manipulation processes, contributing to advancements in modern research.

Long Shelf Life and Quality Assurance

Packaged for optimal stability, Hygromycin B remains effective for up to 24 months when stored unopened. Manufactured in India, it meets rigorous quality standards set by researchers for consistency and dependability in laboratory settings.

FAQ's of Hygromycin B, Phytopure, 1gm:

Q: How is Hygromycin B, Phytopure typically used in laboratories?

A: Hygromycin B, Phytopure is predominantly used as a selection agent during gene transfer experiments, allowing researchers to identify and propagate genetically modified bacteria or plant cells that possess resistance to this antibiotic.

Q: What are the benefits of using this particular Hygromycin B formulation?

A: This product offers high purity, water solubility, and low endotoxin levels, which minimize cellular stress and enhance compatibility with sensitive systems, ensuring accurate selection without compromising cell viability.

Q: When should I use Hygromycin B in my gene transfer protocol?

A: It is best introduced after the transformation or gene transfer step, once the cells have recovered. Adding Hygromycin B to the growth medium allows only those cells containing the resistance gene to survive, streamlining the selection process.

Q: Where can I source Hygromycin B, Phytopure in India?

A: You can obtain this antibiotic from authorized manufacturers, suppliers, or traders across India who specialize in providing high-grade reagents for molecular biology research.

Q: What is the recommended process for dissolving Hygromycin B powder?

A: To prepare a working solution, dissolve the desired quantity of Hygromycin B, Phytopure in sterile water, as it is highly water soluble. Mix until completely dissolved, then filter-sterilize if sterility is required for your application.

Q: Is this Hygromycin B suitable for both bacterial and plant systems?

A: Yes, this formulation is designed to be effective for selection in both bacterial cultures and plant tissue, supporting a broad range of gene transfer experiments.

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