About TAE buffer (10x), 1 L
Premixed liquid 10X concentrate. Contains 0.4M TRIS base, 0.4M Acetate, 10mM EDTA, pH 8.0. Used in agarose electrophoresis for separation of nucleic acids, DNA and RNA. Has lower buffer capacity than TBE but double-stranded DNA runs faster in TAE buffer.
High Purity for Consistent ResultsOur TAE Buffer (10x) is manufactured to deliver high purity, critical for sensitive electrophoresis applications. Its precise composition guarantees reproducible results and reduces the risk of contamination, making it suitable for both research and industrial laboratory environments.
Long Shelf Life and Easy StorageSupplied in a 1 L liquid form, this buffer offers a shelf life of one year, ensuring readiness for frequent laboratory usage. Storing the buffer in a cool and dry place preserves its quality and effectiveness throughout its intended lifespan.
Versatility in UseThe TAE Buffer (10x) is specifically designed for culture media and electrophoresis in molecular biology. It is widely utilized by manufacturers, suppliers, and traders within the industrial lab chemical sector in India, meeting the rigorous demands of modern scientific workflows.
FAQ's of TAE buffer (10x), 1 L:
Q: How should I use TAE Buffer (10x) in agarose gel electrophoresis?
A: To use TAE Buffer (10x) for agarose gel electrophoresis, dilute it to 1x concentration with distilled water before preparing the agarose gel and running buffer. This ensures optimal ionic strength for DNA separation.
Q: What are the benefits of using high-purity TAE Buffer (10x) in the lab?
A: High-purity TAE Buffer minimizes background interference during electrophoresis, leads to clearer DNA bands, and helps maintain consistent migration patterns, ultimately supporting reliable experimental results.
Q: When should the TAE Buffer (10x) be replaced or discarded?
A: It is recommended to use the buffer within its one-year shelf life. Discard any buffer that shows signs of contamination, precipitation, or color change, even within the shelf life period, to maintain experiment integrity.
Q: Where should TAE Buffer (10x), 1 L, be stored for best results?
A: Store the TAE Buffer in a cool and dry location, away from direct sunlight and moisture, to preserve its stability and effectiveness for up to one year.
Q: What is the primary usage of TAE Buffer (10x) in molecular biology laboratories?
A: TAE Buffer (10x) is primarily used for preparing and running agarose gels in nucleic acid electrophoresis as well as in culture media, facilitating DNA separation and analysis.
Q: How is TAE Buffer (10x) supplied, and who can use it?
A: The buffer is supplied as a liquid in 1 L containers and is suitable for manufacturers, suppliers, and traders involved in industrial lab chemicals, including research and educational institutions across India.