Coomassie Brilliant Blue R-250

Coomassie Brilliant Blue R-250 Specification

CAS No
6104-59-2

Physical State
Powder

Storage Instructions
Cool & Dry Place

Purity
High

Usage
Hematology | Staining

Type
Industrial Lab Chemicals

Coomassie Brilliant Blue R-250 Trade Information

Minimum Order Quantity
1 Kilograms

Delivery Time
5-10 Days

About Coomassie Brilliant Blue R-250

Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates. Coomassie Blue R-250 is additionally used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates. A fast acting, sensitive dye which can be used on SDS gels, IEF gels, and standard gels.

Excellent Protein Staining Sensitivity

Coomassie Brilliant Blue R-250 delivers high sensitivity for protein detection in various analytical techniques, especially SDS-PAGE. Its robust staining capabilities enhance band clarity, making it an industry standard for precise protein analysis.

Easy Application and Reliable Results

This powdered dye dissolves readily in aqueous solutions, facilitating hassle-free preparation and consistent staining routines. Researchers and technologists appreciate its quick action and reproducible results, contributing to streamlined laboratory workflows.

FAQ's of Coomassie Brilliant Blue R-250:

Q: How should Coomassie Brilliant Blue R-250 be stored to maintain its quality?

A: Store Coomassie Brilliant Blue R-250 in a cool and dry place, away from direct sunlight or moisture, to preserve its high purity and efficacy over extended periods.

Q: What are the primary applications of Coomassie Brilliant Blue R-250 in the laboratory?

A: Primarily, Coomassie Brilliant Blue R-250 is used for staining proteins after electrophoresis, making it indispensable in hematology and other protein analysis workflows.

Q: When is Coomassie Brilliant Blue R-250 most commonly used during laboratory processes?

A: It is typically employed after gel electrophoresis procedures, where it highlights protein bands for visualization and documentation.

Q: Where can laboratories in India acquire Coomassie Brilliant Blue R-250?

A: This chemical is available from reputable manufacturers, suppliers, and traders across India, ensuring prompt delivery and quality assurance.

Q: What is the process for using Coomassie Brilliant Blue R-250 in protein staining?

A: Dissolve the powdered dye in an appropriate solvent to create a staining solution, then incubate the gel or sample in this solution to achieve distinct and permanent protein band coloration.

Q: What are the key benefits of using this dye for hematology and research applications?

A: It provides high sensitivity, clear visualization, and reproducibility, helping professionals obtain reliable data from routine and critical laboratory analyses.

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