Dl Dithiothreitol, 5gm

Dl Dithiothreitol, 5gm Specification

Packaging Type
Powder

CAS No
27565-41-9

Grade
Molecular Biology Grade

Melting Point
40-44°C

Purity
>99%

Storage Instructions
Store below 0°C

Molecular Weight
154.25 g/mol

Stability
Light sensitive

Size
5 gm

Sensitivity
Air sensitive

Molecular Formula
C4H10O2S2

Odor
Slight sulfurous

Physical State
Solid

Solubility
Soluble in water

Appearance
White crystalline powder

Type
Reducing agent

Shelf Life
36 months

About Dl Dithiothreitol, 5gm

DL-Dithiothreitol is used for stabilizing sulfhydryl containing enzymes. It is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.

Superior Reducing Agent for Molecular Biology

DL Dithiothreitol (DTT) outperforms many traditional reducing agents due to its high purity and molecular biology grade specification. It functions by cleaving disulfide bonds, making it indispensable for protein and enzyme research. Its reliable solid-state and water solubility ensure consistent results in a wide array of analytical and preparative protocols.

Optimal Handling and Storage Guidelines

Due to its sensitivity to air and light, DTT should be handled quickly and stored below 0C after opening. This maximizes the product's shelf life of 36 months. Packaging as a powder in 5g increments allows for precise dispensing and minimal waste in laboratory workflows.

FAQ's of Dl Dithiothreitol, 5gm:

Q: How should DL Dithiothreitol, 5gm be properly stored to maintain stability?

A: It is recommended to store DL Dithiothreitol below 0C in a tightly sealed container, away from light and air, to maintain its stability and extend shelf life up to 36 months.

Q: What are the key benefits of using Dithiothreitol as a reducing agent in molecular biology?

A: Dithiothreitol efficiently reduces disulfide bonds in proteins and enzymes, helping to maintain them in a reduced state, which is vital for accurate experimental results in molecular biology applications.

Q: When should Dithiothreitol be added during experimental procedures?

A: Dithiothreitol is typically added at the start of the procedure or protein preparation to preserve the reduced state of thiol groups and prevent oxidation throughout the experiment.

Q: Where is this Dithiothreitol product available?

A: This Dithiothreitol, packaged as a 5gm white crystalline powder, is available from authorized manufacturers, suppliers, and traders across India.

Q: What is the process for dissolving Dithiothreitol for laboratory use?

A: To prepare a Dithiothreitol solution, dissolve the desired amount in water until fully dissolved, taking care to minimize exposure to air and light to preserve its effectiveness.

Q: Is DL Dithiothreitol suitable for all types of molecular biology experiments?

A: Yes, as a molecular biology grade reagent with high purity, it is appropriate for a wide range of experiments requiring reducing conditions, such as DNA or protein analysis.

Q: What precautions should be taken when handling this product?

A: Wear appropriate protective gear, work quickly to limit exposure to air and light, and always handle in a well-ventilated area due to its slight sulfurous odor and sensitivity.

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