MEM with Earles Salts, w/o L-Glutamine

MEM with Earles Salts, w/o L-Glutamine Specification

CAS No
091212954

Storage Instructions
+2 DegreeC to +8 DegreeC

Physical State
Liquid

Size
500 mL

Packaging Type
Bottle

About MEM with Earles Salts, w/o L-Glutamine

MEM (Minimum Essential Medium) with Earle's Salts was originally developed by Harry Eagle and from there modified in several ways (e.g. alpha modification) to suit many cell culture applications. It is used for cultivation of numerous suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This modification of MEM with Earle's Salts comes without L-Glutamine.

Optimized Formulation for Cell Growth

MEM with Earles Salts (w/o L-Glutamine) is specifically developed to support mammalian cell lines, providing essential nutrients for optimal proliferation and maintenance. Its carefully balanced salt composition helps maintain healthy cellular environments during in vitro experiments.

Reliable Packaging and Storage

Packaged in a sturdy 500 mL bottle, this liquid medium ensures easy handling and accurate pouring. It must be stored between +2C and +8C to preserve its quality, preventing degradation and ensuring experimental consistency throughout its shelf life.

FAQ's of MEM with Earles Salts, w/o L-Glutamine:

Q: How should MEM with Earles Salts, without L-Glutamine, be stored to maintain its effectiveness?

A: This cell culture medium must be stored at temperatures between +2C and +8C to preserve its integrity and prevent degradation. Proper refrigeration is crucial for maintaining its optimal performance over time.

Q: What is the main application of MEM with Earles Salts, w/o L-Glutamine?

A: MEM with Earles Salts is primarily used for cultivating various mammalian cell lines in laboratory settings. It provides essential nutrients and ions required for healthy cell growth in research and production processes.

Q: When is it appropriate to use MEM without L-Glutamine for cell culture work?

A: It is suitable for scenarios where the addition of L-Glutamine is controlled separately, allowing researchers to customize glutamine levels based on specific experimental requirements or cell type sensitivities.

Q: Where is this MEM with Earles Salts product manufactured and available for supply?

A: This medium is manufactured, supplied, and traded in India, and is available to laboratories and research facilities across the region.

Q: What is the recommended process for using MEM with Earles Salts in cell culture experiments?

A: Upon receipt, verify the bottle's physical integrity and expiration date. Equilibrate the medium to room temperature before use, and supplement with L-Glutamine as needed depending on the experimental protocol.

Q: What benefit does a bottle packaging provide for MEM with Earles Salts, w/o L-Glutamine?

A: Bottle packaging ensures sterile and convenient dispensing, reducing contamination risk and enabling accurate measurement during cell culture preparation.

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