DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate

DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate Specification

Physical State
Liquid

Packaging Type
Bottle

Storage Instructions
+2 DegreeC to +8 DegreeC

Size
500 mL

CAS No
091248254

About DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate

DMEM High Glucose (Dulbecco's Modified Eagle Medium) is a standard liquid growth medium that provides basic nutrients for all cell culture applications. This formulation of DMEM comes without L-Glutamine and with Sodium Pyruvate. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium. DMEM supports the growth of various mammalian cells. It can be used to cultivate primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, HEK 293, Cos-7, and PC-12.

Optimized Cell Culture Medium

DMEM High Glucose, without L-Glutamine but containing sodium pyruvate, is designed to support robust cell proliferation, particularly of fast-growing cell lines. Its high glucose content caters to cells requiring elevated energy, while the presence of pyruvate aids metabolic functions. Researchers appreciate its suitability for experiments needing controlled glutamine addition.

Packaging and Storage Assurance

Supplied in a durable 500 mL bottle, this medium ensures consistency and minimizes contamination risk. Adherence to recommended storage conditions (+2C to +8C) preserves efficacy, maintaining optimal support for cell cultures through extended research timelines.

Usage and Application in Research

Primarily used in academic and industrial laboratories throughout India, this DMEM formulation is ideal for cultivating mammalian cells. Its well-balanced composition supports diverse research activities including genetic engineering, drug discovery, and regenerative medicine.

FAQ's of DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate:

Q: How do I properly store DMEM High Glucose (4.5 g/l) without L-Glutamine with sodium pyruvate?

A: Store the DMEM bottle at temperatures between +2C to +8C in a refrigerator. Avoid repeated freezing and thawing, and use within the suggested shelf life to ensure reliability for cell culture applications.

Q: What is the benefit of using DMEM High Glucose with sodium pyruvate for cell cultures?

A: This medium provides enhanced energy support due to the high glucose concentration, while sodium pyruvate contributes to cellular metabolism. It is highly suitable for culturing fast-growing mammalian cells, ensuring robust proliferation and viability.

Q: When should researchers opt for DMEM without L-Glutamine?

A: Researchers may select DMEM without L-Glutamine when they need precise control of glutamine concentration in their experiments, such as studying glutamine metabolism or regulating supplement addition tailored to specific cell requirements.

Q: Where can I purchase DMEM High Glucose (4.5 g/l) with sodium pyruvate in India?

A: This product is available from multiple manufacturers, suppliers, and traders across India. Contact local laboratory supply companies or visit relevant scientific marketplaces to acquire the 500 mL bottle packaging.

Q: What is the recommended process for using this DMEM in cell culture applications?

A: To use, first warm the medium to room temperature before introducing it to cell cultures. Supplement with L-Glutamine if required. Regularly monitor cell growth and replace medium as necessary for optimal results.

Q: Is this DMEM formulation ready to use, or do I need to add further supplements before culturing cells?

A: While the medium contains high glucose and sodium pyruvate, it does not include L-Glutamine. Supplement with L-Glutamine and other necessary additives according to your cell line's requirements before use.

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