DNA pro Kit for Soil

DNA pro Kit for Soil
DNA pro Kit for Soil

DNA pro Kit for Soil Specification

  • Size
  • 10 Preps
  • CAS No
  • 116549010
  • Storage Instructions
  • Store at Room Temperature (15-25 Degree Celsius), Dry environment
 
 

About DNA pro Kit for Soil

  • Key features and detailsSoil samples are a complex environment characterized by large variations of microbial load and diversity that prove to be challenging to analyze due to the presence of some inhibitory compounds such as humic acid, heavy metals, and other aromatic components.
  • Product Description

    Soil samples are a complex environment characterized by large variations of microbial load and diversity that prove to be challenging to analyze due to the presence of some inhibitory compounds such as humic acid, heavy metals, and other aromatic components.

    The SPINeasy DNA Maxi Kit for Soil has been optimized to enable fast and convenient isolation of pure microbial DNA from large amount of soil. All types of soil samples are compatible with this kit, including those with high microbial load and humic acid content such as compost (up to 10g), or those with low microbial load such as sand (up to 20g). The SPINeasy DNA Maxi Kit for Soil effectively lyses various microbiome population such as bacteria, fungi, viruses, protists with reduced processing time. The isolated high purity DNA is fully compatible with downstream PCR and NGS applications (16S and whole genome) without the need for further inhibitor removal steps.

    Performance

    The SPINeasy DNA Maxi Kit for Soil has been extensively tested across a wide range of soil types and starting amount. The isolated DNA has high purity, as demonstrated by the optimal A260/280 and A260/230 ratios. Unlike competitor Q, the SPINeasy DNA Maxi Kit for Soil can be loaded with larger amount of sample (10 g and 20 g of high and low biomass soil samples respectively), delivering up to 800 g of DNA within a small elution volume (<2 mL). Furthermore, similar yields were repeatably observed when using the same amount of starting sample, indicating an excellent reproducibility.

    Left: gDNA extracted from a wide range of soil samples with different microbial loads and contaminant levels using SPINeasy DNA Maxi Kit for Soil.

    Right: SPINeasy DNA Maxi Kit for Soil demonstrates high reproducibility with great DNA yield and purity when compared to competitor Q.

    Application Notes

    The SPINeasy DNA Maxi Kit for Soil has been optimized to enable fast and efficient isolation of pure microbial DNA from large amount of soil sample. All types of soil samples are compatible with this kit, including those with high microbes and humic acid content such as compost (up to 10g) or those with low microbes content such as sand (up to 20g).

    Key Applications

    Genomic, Molecular Biology, PCR, qPCR



Efficient Soil DNA Extraction

Extracting DNA from soil can be challenging due to complex sample matrices. This kit offers a reliable solution, allowing users to obtain pure DNA suitable for downstream molecular applications, including PCR, sequencing, and microbial analysis.


Convenient Room Temperature Storage

Forget the hassle of refrigeration. The DNA pro Kit for Soil is formulated for stability and activity at room temperature, between 15 and 25C, ensuring convenience and flexibility in any laboratory or field setting.


Applications in Research and Agriculture

From academic soil ecology studies to applied agricultural biotechnology, this kit enables rapid, reproducible DNA extraction from various soils. It accelerates workflows in microbial biodiversity assessment and environmental monitoring projects.

FAQ's of DNA pro Kit for Soil:


Q: How do I use the DNA pro Kit for Soil to extract DNA from my samples?

A: Simply follow the provided protocol, which involves disrupting the soil sample, binding DNA to the included matrix, washing away impurities, and eluting pure DNA. The kit includes all necessary reagents for up to 10 extractions.

Q: What types of soil samples are compatible with this kit?

A: The kit is compatible with a wide range of soil types, including agricultural, environmental, and garden soils, making it versatile for various research and analytical applications.

Q: When should I use the DNA pro Kit for Soil?

A: Use this kit whenever you require efficient extraction of high-quality DNA from soil samples for further analysis, such as microbial diversity studies, PCR, or sequencing.

Q: Where should I store the DNA pro Kit for Soil?

A: Store the kit at room temperature (15-25C) in a dry environment as instructed. Avoid exposure to excessive humidity to maintain reagent integrity.

Q: What is the process involved in the DNA extraction with this kit?

A: The process includes sample lysis, DNA binding, washing steps, and final elution, all streamlined with ready-to-use solutions to ensure quick and effective DNA recovery.

Q: What are the benefits of using this kit in my laboratory or fieldwork?

A: Benefits include efficient DNA extraction, time savings, convenient room-temperature storage, and reliable, reproducible results, making it ideal for both high-throughput labs and fieldwork situations.

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