DNA pro Kit for Soil

About DNA pro Kit for Soil

• Key features and detailsSoil samples are a complex environment characterized by large variations of microbial load and diversity that prove to be challenging to analyze due to the presence of some inhibitory compounds such as humic acid, heavy metals, and other aromatic components.
• Product Description

  Soil samples are a complex environment characterized by large variations of microbial load and diversity that prove to be challenging to analyze due to the presence of some inhibitory compounds such as humic acid, heavy metals, and other aromatic components.

  The SPINeasy DNA Maxi Kit for Soil has been optimized to enable fast and convenient isolation of pure microbial DNA from large amount of soil. All types of soil samples are compatible with this kit, including those with high microbial load and humic acid content such as compost (up to 10g), or those with low microbial load such as sand (up to 20g). The SPINeasy DNA Maxi Kit for Soil effectively lyses various microbiome population such as bacteria, fungi, viruses, protists with reduced processing time. The isolated high purity DNA is fully compatible with downstream PCR and NGS applications (16S and whole genome) without the need for further inhibitor removal steps.

  Performance

  The SPINeasy DNA Maxi Kit for Soil has been extensively tested across a wide range of soil types and starting amount. The isolated DNA has high purity, as demonstrated by the optimal A260/280 and A260/230 ratios.  Unlike competitor Q, the SPINeasy DNA Maxi Kit for Soil can be loaded with larger amount of sample (10 g and 20 g of high and low biomass soil samples respectively), delivering up to 800 g of DNA within a small elution volume (<2 mL). Furthermore, similar yields were repeatably observed when using the same amount of starting sample, indicating an excellent reproducibility.

   

  Left: gDNA extracted from a wide range of soil samples with different microbial loads and contaminant levels using SPINeasy DNA Maxi Kit for Soil.

  Right: SPINeasy DNA Maxi Kit for Soil demonstrates high reproducibility with great DNA yield and purity when compared to competitor Q.

  Application Notes

  The SPINeasy DNA Maxi Kit for Soil has been optimized to enable fast and efficient isolation of pure microbial DNA from large amount of soil sample. All types of soil samples are compatible with this kit, including those with high microbes and humic acid content such as compost (up to 10g) or those with low microbes content such as sand (up to 20g).

  Key Applications

  Genomic, Molecular Biology, PCR, qPCR