Plasmid Midiprep Kit

About Plasmid Midiprep Kit

• Product Description

  SPINeasy Plasmid Midiprep Kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 50 mL of transformed bacterial culture. Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the lysate supernatant is adsorbed on a silica membrane, washed and eluted in a convenient process using the spin column technology. Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology laboratory applications.

  Kit performance

   

  Figure 1: Agarose gel electrophoresis result of pcDNA3.1 plasmid DNA extracted using the SPINeasy Plasmid Midiprep Kit with downstream molecular applications. M: DNA marker; Lane 1: Purified plasmid DNA; Lane 2: Plasmid digested with two single cut restriction enzymes, yielding two bands; Lane 3: PCR of plasmid, producing a band of the expected amplicon size; Lane 4: PCR negative control.

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  Application Notes

  The SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 50 mL of transformed bacterial cultures.

  Usage Statement

  Research Use Only (RUO).

  Key Applications

  In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, Genomics, Molecular Biology