Plant DNA Extraction Kit

Plant DNA Extraction Kit Specification

Size
50 Preps

CAS No
116535050

Storage Instructions
Store at Room Temperature (15-30 Degree celsius) in a dry environment

Shelf Life
12 months

Time Required
30 minutes per prep

Operation
Simple protocol

Yield Efficiency
High DNA yield

Kit Type
Plant DNA extraction

Pack Includes
Buffers, spin columns

Hazard Information
Non-toxic kit

Purity Level
PCR compatible

Format
Column-based purification

Sample Compatibility
Leaf, seed, stem

About Plant DNA Extraction Kit

Key features and details
Easy isolation of genomic DNA from plant using spin column technology

Product Description

SPINeasy DNA Kit for Plant is a silica-membrane spin-column kit that enables quick, convenient and cost-effective isolation of genomic DNA (gDNA) from various plant tissues, such as leaf, stem and seed. High quality gDNA can be extracted from polysaccharide and polyphenolic-rich plants. The use of our specially formulated Lysis Buffer PD and Lysing Matrix A in combination with FastPrep Instruments from MP Biomedicals enables highly efficient lysis of various types of samples within seconds. For DNA purification, Column PD and kit buffers are designed to deliver gDNA extracts of high yield and purity which are compatible with downstream molecular biology applications such as PCR, restriction digestion and sequencing.

Kit performance

	Figure 1 (left): gDNA extracted from various samples using SPINeasy DNA Kit for Plant, analyzed using agarose gel electrophoresis. M: DNA marker; Lane 1: Pothos leaf; Lane 2: Tomato leaf; Lane 3: Chili leaf; Lane 4: Tobacco leaf; Lane 5: Pine needle; Lane 6: Corn leaf; Lane 7: Corn kernel.

Figure 2 (right): PCR amplification of gDNA extracted from various samples using SPINeasy DNA Kit for Plant. M: DNA marker; Lane 1: Pothos leaf; Lane 2: Tomato leaf; Lane 3: Chili leaf; Lane 4: Tobacco leaf; Lane 5: Pine needle; Lane 6: Corn leaf; Lane 7: Corn kernel; Lane 8: Negative control

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Application Notes

Deliver gDNA extracts of high yield and purity from plant tissues that are compatible with downstream applications such as PCR, restriction digestion and sequencing.

Usage Statement

Research Use Only (RUO).

Key Applications

Plant Genomics, Molecular Biology, PCR, NGS

Efficient Column-Based Purification

Achieve high DNA yield and purity with a robust column-based system. This method efficiently removes contaminants, delivering DNA ready for sensitive applications such as PCR. Its design ensures reproducible results across various plant sample types, making it a favorite among researchers and professionals.

Versatile Plant Sample Compatibility

Whether working with leaves, seeds, or stems, this kit accommodates multiple plant tissue types. The protocol has been optimized to provide consistent extraction performance regardless of sample origin, streamlining genetic studies and plant research workflows.

Quick and Safe Preparation

Complete the DNA extraction process in just 30 minutes per sample. The kit utilizes a non-toxic composition for safe handling and disposal, prioritizing both laboratory safety and environmental responsibility. Instructions are straightforward, enabling both novices and experienced users to obtain optimal results.

FAQ's of Plant DNA Extraction Kit:

Q: How does the Plant DNA Extraction Kit simplify the DNA isolation process?

A: This kit features a straightforward protocol with all necessary buffers and spin columns included. Its column-based purification eliminates contaminants quickly, allowing users to extract high-quality plant DNA efficiently and safely.

Q: What types of plant samples are compatible with the extraction kit?

A: The kit is designed for a wide range of plant tissues, including leaves, seeds, and stems. This versatility allows for flexibility across various research and testing applications.

Q: When should the kit be used within its shelf life for best results?

A: For optimal performance, it is recommended to use the kit within its 12-month shelf life. Proper storage at room temperature in a dry environment ensures reagent stability and effectiveness.

Q: Where should the Plant DNA Extraction Kit be stored?

A: Store the kit at room temperature (15-30C) in a dry setting. Avoid exposing the components to excessive moisture or extreme temperatures to maintain the kit's integrity.

Q: What is the process for using the Plant DNA Extraction Kit?

A: Simply prepare your plant sample, add the provided buffers, and use the spin columns to purify the DNA. The process takes approximately 30 minutes per sample and requires minimal equipment, making it suitable for most labs.

Q: Is the DNA extracted with this kit suitable for PCR applications?

A: Yes, the kit is specifically designed to yield DNA of purity levels compatible with PCR and other downstream molecular biology applications, ensuring reliable results.

Q: What are the main benefits of using this kit for plant DNA extraction?

A: Users benefit from high DNA yield, rapid processing time, broad sample compatibility, non-toxic reagents, and a protocol that's simple and reproducible, making it ideal for routine and advanced plant molecular studies.

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