About MPure-32 MagBeads Fast Circulating DNA Kit
MagBeads Fast Circulating DNA Kit is intended for purification of high-quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum) using the MPure-32 aNAP System. The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, Biochip analysis and NGS.
Efficient Extraction for Liquid BiopsyExtracting cell-free DNA from plasma or serum is essential for applications like liquid biopsies. The MPure-32 MagBeads Fast Circulating DNA Kit delivers high yields by pairing advanced magnetic bead technology with fully automated processing on the MPure-32 system, ensuring consistent and pure DNA for sensitive downstream analysis.
Streamlined Workflow, Maximum ThroughputThis kit supports a robust workflow, enabling the simultaneous processing of up to 32 samples per run. Each extraction takes approximately 30 to 60 minutes, minimizing hands-on time while maximizing productivity in research or clinical labs handling circulating DNA analysis.
FAQ's of MPure-32 MagBeads Fast Circulating DNA Kit:
Q: How does the MPure-32 MagBeads Fast Circulating DNA Kit extract DNA from blood plasma or serum?
A: The kit utilizes magnetic bead-based technology to selectively capture and purify circulating DNA, including ctDNA and cfDNA, from plasma or serum samples. The automated MPure-32 system processes up to 32 samples per run, ensuring uniform extraction and high-purity results.
Q: What is the recommended process for using this kit in a laboratory setting?
A: Simply load the blood plasma or serum samples into the MPure-32 system along with the ready-to-use reagents from the kit. The automated process guides extraction, eluting DNA in 50-100 L volumes suitable for downstream qPCR or NGS applications.
Q: When should I choose this kit for my DNA extraction protocols?
A: This kit is ideal when you need efficient, high-throughput extraction of circulating DNA with high yield and quality-especially for applications involving sensitive analyses like liquid biopsy, cancer genomics, or other NGS/qPCR-based tests.
Q: Where should the MPure-32 MagBeads Fast Circulating DNA Kit be stored?
A: For optimal performance and longevity, the kit should be stored at refrigerated temperatures between 2-8C. This maintains reagent stability and ensures consistent results up to its minimum one-year shelf life.
Q: What are the primary benefits of using this kit compared to manual extraction methods?
A: This kit streamlines extraction with automated processing, significantly reducing hands-on time while increasing reproducibility and minimizing sample-to-sample variation. Its magnetic bead technology also delivers high yields of pure DNA suitable for high-sensitivity applications.
Q: Can this kit be used with sample types other than blood plasma or serum?
A: The MPure-32 MagBeads Fast Circulating DNA Kit is optimized specifically for blood plasma and serum samples. Using other sample types is not recommended, as it may affect DNA yield and purity.