About Guanidine Thiocyanate
Guanidine Thiocyanate is often used in the isolation of intact ribonucleic acid to eliminate RNase activity. A 4 M solution of guanidine thiocyanate irreversibly inactivates RNase. In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost.
Versatile Laboratory ReagentGuanidine Thiocyanate is vital in research settings for its role in disrupting cellular membranes, making it essential for processes such as RNA and DNA extraction. Its consistency and high purity make it a top choice among professionals involved in genomics and proteomics studies.
Reliable Quality and PackagingSupplied in a 500 gm bottle with 98% purity, this laboratory-grade chemical upholds high standards required by researchers. Careful packaging protects against contamination and degradation, ensuring dependable results in sensitive experiments.
FAQ's of Guanidine Thiocyanate:
Q: How is Guanidine Thiocyanate typically used in the laboratory?
A: Guanidine Thiocyanate is primarily used for nucleic acid extraction, where it denatures proteins and lyses cells, helping to isolate DNA and RNA efficiently. Its strong chaotropic properties make it essential in molecular biology protocols.
Q: What processes benefit from using Guanidine Thiocyanate of 98% purity?
A: Processes such as gene expression analysis, viral RNA extraction, and protein denaturation benefit greatly from the high purity (98%) of Guanidine Thiocyanate, as impurities could compromise data quality and reproducibility.
Q: When should researchers choose research grade Guanidine Thiocyanate?
A: Researchers should opt for research grade Guanidine Thiocyanate when conducting sensitive experiments requiring consistent and reliable results, especially in genomics, cell biology, and protein studies.
Q: Where can I procure Guanidine Thiocyanate in India?
A: You can acquire Guanidine Thiocyanate from manufacturers, suppliers, or traders specializing in laboratory chemicals in India. Ensure that the source provides the product with proper documentation and research grade assurance.
Q: What are the benefits of using the 500 gm bottle packaging for laboratory use?
A: The 500 gm bottle is practical for research as it offers a sufficient quantity for multiple experiments, reduces the frequency of reordering, and provides secure, contamination-resistant storage to maintain chemical integrity.
Q: How does Guanidine Thiocyanate improve molecular biology workflows?
A: Guanidine Thiocyanate enhances molecular biology workflows by providing rapid and effective cell lysis and protein denaturation, resulting in high-quality nucleic acid and protein samples suitable for downstream analysis.