Proteinase K Enzyme, 25mg

Proteinase K Enzyme, 25mg Specification

Physical State
Powder

CAS No
39450-01-6

Solubility
Water Soluble

Stability
Long Term

Activity
>30 Units/mg

Color
White Lyophilized

About Proteinase K Enzyme, 25mg

Proteinase K is useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. It is also useful in removal of endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. It is reported to be useful for the isolation of hepatic, yeast, and mung bean mitochondria. It is useful in determination of enzyme localization on membranes. It is also used in treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Proteinase K is used in digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Superior Enzymatic Performance

Proteinase K delivers robust protein digestion owing to its high activity levels exceeding 30 units/mg. It is ideal for breaking down proteins in biological samples, supporting various laboratory workflows such as DNA or RNA extraction and analysis. Its dependable activity streamlines research and diagnostic processes.

Ease of Solubility and Application

Formulated for optimal water solubility, Proteinase K is simple to dissolve and integrate into laboratory protocols. Its white, lyophilized powder form allows for accurate measurement and easy handling. The enzyme is well-suited for use in DNA, RNA, and protein sample preparation.

Reliability and Stability

With superior long-term stability, this enzyme maintains activity levels over extended storage. Its stable properties ensure consistent results throughout ongoing use, making it a valuable resource for research facilities, diagnostic labs, and industrial applications.

FAQ's of Proteinase K Enzyme, 25mg:

Q: How can I dissolve Proteinase K Enzyme, 25mg for use in experiments?

A: Proteinase K is highly water soluble. To prepare, simply add the desired amount of powder to distilled water or buffer and mix gently until fully dissolved. This allows easy integration into your laboratory protocols.

Q: What are the primary applications of this enzyme in laboratory settings?

A: This enzyme is widely used for the digestion of proteins during DNA and RNA purification procedures, cell lysis, and protein removal from biological samples, making it an essential tool in molecular biology and genomic research.

Q: When should I use Proteinase K during the nucleic acid extraction process?

A: Proteinase K should be added after cell lysis but before other purification steps. Its activity ensures thorough protein removal, which enhances downstream nucleic acid yield and purity.

Q: Where is this Proteinase K Enzyme sourced and manufactured?

A: Our Proteinase K is manufactured and supplied by reputable producers in India, ensuring high quality and consistent availability for research and industrial needs.

Q: What is the process for storing Proteinase K to maintain its long-term stability?

A: Store the white lyophilized powder in a tightly sealed container at recommended refrigerated or room temperature conditions. This helps preserve its enzymatic activity and long-term performance.

Q: How does the >30 Units/mg activity benefit my research applications?

A: The high activity means you require less enzyme for efficient protein digestion, improving workflow efficiency and reducing costs without compromising the quality of results.

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