Yeast DNA Extraction Kit

Yeast DNA Extraction Kit Specification

CAS No
116557050

Storage Instructions
Store at Room Temperature (15-25 Degree celsius), Dry environment.

Appearance
White fine powder

Usage
Laboratory use

Grade
Molecular Biology Grade

Type
Extraction Kit

Density
Not determined

Packaging Type
Sealed box

Ph Level
7.5

Purity(%)
>99

Application
Yeast gDNA isolation

Shelf Life
12 Months

Physical State
Solid

Size
100 Preps

About Yeast DNA Extraction Kit

Key features and details
SPINeasy DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes.
Product Description
Yeast cell walls are resilient, hence it can become a challenge when trying to extract the intact purified DNA. Many times, the extracted DNA can be damaged or contaminated due to unintuitive workflow. SPINeasy DNA Kit for Yeast is a high-performance genomic DNA (gDNA) extraction kit which is developed based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from yeast cells, typically in less than 30 minutes. Provided in the kit, Column S and kit buffers are designed to deliver gDNA extracts of high yield and purity; the extracted gDNA is compatible with downstream applications such as PCR amplification, restriction enzyme digestion, and sequencing.
Performance

Figure 1. SPINeasy DNA Kit for Yeast generates DNA yield that is proportionate to the input sample amount, Saccharomyces cerevisiae (1 x 107 to 4 x 108 cells). 3 L of purified genomic DNA from a 100 L eluate were analyzed by electrophoresis on a 1% agarose gel.

Figure 2.

The SPINeasy DNA Kit for Yeast was used to extract genomic DNA (gDNA) from Saccharomyces cerevisiae. The yield and purity were assessed and compared with three different competitor kits (Q, T, Z). The MP Kit exhibited a purity range exceeding 1.8 while maintaining a high yield. Competitors Q and T yielded high quantities of gDNA but with a lower A260/A230 ratio. Competitor Z yielded both a lower quantity of gDNA and a lower purity ratio. Gel electrophoresis of the extracted gDNA displays maximum band integrity for the MP Kit.
Application Notes
The SPINeasy DNA Kit for Yeast was designed for rapidly isolating high-quality DNA from yeast samples.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
PCR amplification, restriction enzyme digestion, and sequencing

Efficient Yeast Genomic DNA Isolation

Designed for fast and reliable extraction, this kit allows users to isolate yeast genomic DNA with minimal hands-on time. The high purity level of over 99% ensures suitability for sensitive downstream molecular biology applications, including PCR, cloning, and sequencing.

User-Friendly Laboratory Application

The Yeast DNA Extraction Kit arrives as a white, fine powder in a sealed box, ready for immediate laboratory use. Just follow the provided protocols to achieve consistent yields from up to 100 yeast samples, making it a practical choice for busy research environments.

Optimal Storage and Longevity

Maintain the kit's integrity by storing it at room temperature (15-25C) in a dry environment. With a shelf life of 12 months, the kit remains stable and ready for use, reducing waste and improving laboratory efficiency.

FAQ's of Yeast DNA Extraction Kit:

Q: How do I use the Yeast DNA Extraction Kit for isolating yeast genomic DNA?

A: To use the kit, follow the manufacturer's protocol provided in the sealed box. Add the powder to yeast cell samples as instructed, process according to the outlined steps, and you will efficiently isolate high-purity genomic DNA.

Q: What are the benefits of using this molecular biology grade extraction kit?

A: The kit offers rapid, reliable extraction of yeast gDNA at over 99% purity, supporting accurate results in molecular biology analyses such as PCR or sequencing. It also features user-friendly handling and consistent performance across 100 preparations.

Q: When should I replace the Yeast DNA Extraction Kit?

A: It is recommended to use the kit within its shelf life of 12 months. Replace the kit once it reaches its expiration to ensure optimal DNA yield and purity for your experiments.

Q: Where can the kit be stored to maintain its quality?

A: Store the kit at room temperature (15-25C) in a dry environment, as detailed by the manufacturer. Avoid exposure to moisture or extreme temperatures to preserve its quality and effectiveness.

Q: What is the process for extracting DNA using this kit?

A: The kit is supplied as a white fine powder that is added to yeast samples. Following a sequence of mixing, incubation, and separation steps according to the enclosed protocol enables efficient extraction of genomic DNA from yeast cells.

Q: Can this kit be used for applications beyond yeast gDNA isolation?

A: This kit is specifically designed and optimized for yeast genomic DNA isolation in molecular biology settings. For other applications, consult with your supplier or manufacturer for appropriate alternatives.

Q: What makes this yeast DNA extraction kit suitable for laboratory use in India?

A: The kit meets high purity standards, is available through certified manufacturers, suppliers, and traders in India, and is packaged for safe transport and easy incorporation into routine laboratory workflows.

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