Ribonuclease A (Bovine)

Ribonuclease A (Bovine) Specification

Storage Instructions
-0 DegreeC, desiccate

Physical State
Powder

Ph Level
Optimum pH: 7.0 - 7.5

CAS No
9001-99-4

About Ribonuclease A (Bovine)

Ribonuclease A, and to a greater extent its oligomers and some homologs (such as onconase from frogs), have cytotoxic and cytostatic effects, particularly on cancer cells.This attribute has led to the development of onconase as a cancer therapeutic.

Enzymatic Efficiency and Stability

Ribonuclease A (Bovine) exhibits maximum enzymatic activity at a pH between 7.0 and 7.5, making it highly effective for RNA digestion in controlled laboratory reactions. Its powder form ensures prolonged shelf-life when stored as recommended. Proper storage at or below 0C with desiccation is crucial to preserve its functionality and structural stability.

Applications in Biotechnology

This enzyme is widely used in molecular biology laboratories across India for its robust RNA-degrading capability. Its purity and reliability make it an essential reagent in applications such as RNA removal from DNA samples, structure-function studies, and RNA sequencing workflows. Researchers value its consistency and straightforward handling in complex protocols.

FAQ's of Ribonuclease A (Bovine):

Q: How should Ribonuclease A (Bovine) powder be stored to maintain its activity?

A: Ribonuclease A (Bovine) powder should be stored at 0C or below, in a desiccated environment, to ensure the enzyme remains active and stable throughout its shelf-life.

Q: What is the optimum pH for using Ribonuclease A (Bovine) in laboratory protocols?

A: The enzyme exhibits its best activity between pH 7.0 and 7.5, making this range ideal for most experimental procedures requiring efficient RNA degradation.

Q: When is Ribonuclease A (Bovine) most commonly used during laboratory procedures?

A: Ribonuclease A (Bovine) is typically utilized during the removal of RNA contaminants from DNA preparations, during the study of RNA structure, or when precise RNA degradation is required in research workflows.

Q: Where can this enzyme be sourced in India?

A: It can be procured from authorized manufacturers, suppliers, and traders specializing in laboratory reagents and enzymes across India.

Q: What is the process of using Ribonuclease A (Bovine) in RNA-related experiments?

A: The process involves dissolving the powder in an appropriate buffer at pH 7.0-7.5, following which it is added directly to the sample for efficient RNA degradation as per protocol requirements.

Q: What are the main benefits of using Ribonuclease A (Bovine) in molecular biology?

A: The enzyme offers reliable, efficient RNA cleavage, facilitating purification steps in DNA research and other molecular studies, with high stability under recommended storage conditions.

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