Blood DNA Extraction Kit

About Blood DNA Extraction Kit

• Key features and details

  • Rapid and efficient DNA isolation in less than 30 minutes

  • Fresh, frozen, or anticoagulated blood

  • Simple and effective lysis protocol using Lysis Buffer BL and Proteinase K to omit the need of any mechanical lysis

  • No organic extraction

  • High purity DNA ready to use for downstream application

  • Product Description

    SPINeasy DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from other samples, including plasma, serum, saliva, and cell culture medium. The use of our specially formulated Lysis Buffer BL omits the need of homogenization and enables highly efficient lysis of various samples. Provided in the kit, Column S and kit buffers are designed to deliver gDNA extracts of high yield and purity, compatible with downstream applications such as PCR amplification.

    Performance

    SPINeasy DNA Kit for Blood has been tested on various sample types for its performance. The following data demonstrate the gDNA yields obtained from various sample sources. Results showed that all extracted gDNA are of high yield and purity (Table 1, Figure 1, and Figure 2) which suitable for downstream PCR amplification (Figure 3).

    Table 1: Quality and quantity of gDNA extracted from various sample types using SPINeasy DNA kit for Blood.

     

    *Sample processed using homogenization method via Fastprep-24TM 5G with lysing matrix.

    Sample preserved in preservation buffer (SPS, provided in SPINeasy DNA Kit for Saliva)

    Application Notes

    SPINeasy DNA Kit for Blood uses spin column technology for isolation of gDNA from variety of sample sources which include fresh, frozen, and anticoagulated blood, plasma, serum, and cell cultured medium.

    Usage Statement

    Research Use Only (RUO)

    Key Applications

    Genomics; Molecular Biology; PCR, qPCR, Microbiome analysis, NGS; shotgun sequencing