Blood DNA Extraction Kit

Blood DNA Extraction Kit Specification

CAS No
116552050

Storage Instructions
15-25 Degree Celsius

Appearance
Lyophilized powder

Grade
Molecular biology grade

Usage
Genomic DNA isolation

Purity(%)
>99%

Shelf Life
12 months

Ph Level
7.5-8

Application
Molecular biology

Physical State
Solid

Size
50 preps

Type
Blood DNA extraction

Packaging Type
Box

About Blood DNA Extraction Kit

Key features and details
- Rapid and efficient DNA isolation in less than 30 minutes
- Fresh, frozen, or anticoagulated blood
- Simple and effective lysis protocol using Lysis Buffer BL and Proteinase K to omit the need of any mechanical lysis
- No organic extraction
- High purity DNA ready to use for downstream application
- Product Description
  SPINeasy DNA Kit for Blood is a high-performance genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from whole blood preserved in different anticoagulants (EDTA, Heparin and sodium citrate), typically in less than 30 minutes. It also allows easy gDNA isolation from other samples, including plasma, serum, saliva, and cell culture medium. The use of our specially formulated Lysis Buffer BL omits the need of homogenization and enables highly efficient lysis of various samples. Provided in the kit, Column S and kit buffers are designed to deliver gDNA extracts of high yield and purity, compatible with downstream applications such as PCR amplification.
  Performance
  SPINeasy DNA Kit for Blood has been tested on various sample types for its performance. The following data demonstrate the gDNA yields obtained from various sample sources. Results showed that all extracted gDNA are of high yield and purity (Table 1, Figure 1, and Figure 2) which suitable for downstream PCR amplification (Figure 3).
  
  Table 1: Quality and quantity of gDNA extracted from various sample types using SPINeasy DNA kit for Blood.
  
  *Sample processed using homogenization method via Fastprep-24TM 5G with lysing matrix.
  
  Sample preserved in preservation buffer (SPS, provided in SPINeasy DNA Kit for Saliva)
  Application Notes
  SPINeasy DNA Kit for Blood uses spin column technology for isolation of gDNA from variety of sample sources which include fresh, frozen, and anticoagulated blood, plasma, serum, and cell cultured medium.
  Usage Statement
  Research Use Only (RUO)
  Key Applications
  Genomics; Molecular Biology; PCR, qPCR, Microbiome analysis, NGS; shotgun sequencing

Efficient Genomic DNA Isolation

This Blood DNA Extraction Kit delivers rapid and thorough genomic DNA isolation from blood samples. Its lyophilized powder format ensures stability and integrity of reagents, simplifying preparation and minimizing contamination risk in the workflow. Suitable for various downstream applications in molecular biology, it guarantees exceptional DNA yield and purity.

Optimized for Research Consistency

Formulated with molecular biology grade reagents, each kit is crafted to ensure reproducibility across experiments. Its high purity (>99%) and controlled pH (7.5-8) support optimal performance, making it suitable for a wide variety of molecular techniques, such as PCR, sequencing, and genotyping.

FAQ's of Blood DNA Extraction Kit:

Q: How should I store the Blood DNA Extraction Kit to maintain its effectiveness?

A: The kit should be stored at a temperature between 15-25 degrees Celsius in its original packaging to preserve the lyophilized powder and guarantee optimal reagent stability throughout its 12-month shelf life.

Q: What is the main benefit of using this kit for genomic DNA extraction from blood samples?

A: The kit provides high-purity (>99%) genomic DNA with consistent yield, thanks to its molecular biology grade formulation and optimized pH. This purity is crucial for reliable results in downstream applications including PCR, sequencing, and cloning.

Q: When is this kit typically used in the molecular biology workflow?

A: This kit is employed at the initial DNA isolation stage of molecular biology workflows, directly after blood sample collection. Extracted DNA can then be utilized in a wide variety of research and diagnostic assays.

Q: Where is the Blood DNA Extraction Kit manufactured and supplied from?

A: The kit is manufactured, supplied, and traded in India, meeting rigorous quality standards to support both research and diagnostic laboratories.

Q: What is the process for extracting DNA using this kit?

A: Users add the provided lyophilized reagents to their blood samples, follow a series of incubation and washing steps as directed in the kit manual, and ultimately purify genomic DNA suitable for immediate use.

Q: How many extractions does each kit support and what is included in the packaging?

A: One box contains sufficient lyophilized reagents to perform 50 separate DNA extractions (preps), with all necessary buffers and components included for complete genomic DNA isolation from blood samples.

Tell us about your requirement