Microbiome DNA Extraction Kit

Microbiome DNA Extraction Kit Specification

CAS No
116553050

Size
50 Preps

Storage Temperature
Room Temperature

Lysis Buffer Included
Yes

Sample Types
Stool, Soil, Swabs

Elution Volume
50-100 µl

Extraction Time
Under 60 min

Format
Spin Column

DNA Yield
High

Sample Preservation
Yes

Compatibility
Manual, Automation

About Microbiome DNA Extraction Kit

Key features and detailsSPINeasy DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica-membrane spin-column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids and environmental samples such as soil and stool.
Product Description
SPINeasy DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica-membrane spin-column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids and environmental samples such as soil and stool. With the use of specially formulated Buffer MB1 and Lysing Matrix E in combination with FastPrep Instruments from MP Biomedicals, an efficient lysis of various samples can be achieved within seconds. Provided in the kit, the Column MB and kit buffers are designed to deliver gDNA of high yield and purity, and compatible with downstream applications such as qPCR, restriction digestion, and sequencing.
Performance
SPINeasy DNA Kit for Microbiome has been rigorously validated on various types of samples.
Microbial culture samples
The bacterial DNA extracted with SPINeasy DNA Kit for Microbiome has higher yield as compared to that of competitor kits.

Bronchoalveolar lavage fluid (BALF)
The DNA extracted with SPINeasy DNA Kit for Microbiome has high yield and good DNA integrity.
Milk
Different types of milk contain different amounts of protein and fat. SPINeasy DNA Kit for Microbiome has an effective impurity removal technology and is able to extract gDNA from the three types of milk tested.The DNA extracted with SPINeasy DNA Kit for Microbiome showed high yield and better purity (especially for fresh milk) than that of competitor kits.

Human whole blood
The DNA extracted with SPINeasy DNA Kit for Microbiome showed high yield and purity.

Inhibitor-Free DNA Ready for Downstream Applications
The DNA extracted with SPINeasy DNA Kit for Microbiome could be directly used for downstream applications (such as qPCR), suggesting the effectiveness of the kit in removing any inhibitor.

Application Notes
Efficient and unbiased lysis of microbes, including gram positive/negative bacteria, fungi, protozoans, and viruses found in swab, sputum, body fluid, stool, soil, and water samples.

SPINeasy DNA Kit for Microbiome employs a novel method for isolating microbial genomic DNA from various samples. Due to its highly effective lysis capability and silica membrane spin column technology, the SPINeasy DNA Kit for Microbiome can be used for bacterial, body fluids, and environmental samples such as soil and stool.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Genomic, Molecular Biology, PCR, qPCR, Microbiome analysis, NGS, Shotgun sequencing

Efficient and Versatile Extraction

The Microbiome DNA Extraction Kit offers quick, high-performance DNA purification for various samples such as stool, soil, and swabs. Its spin column format streamlines workflows and is compatible with both manual and automated processing, saving time and labor in the lab.

High DNA Yield and Quality

This kit extracts DNA with high efficiency, ensuring sufficient yield for downstream applications. The included optimized lysis buffer and specialized protocol preserve nucleic acid integrity, making it suitable for sensitive microbiome and molecular studies.

Convenient and Reliable Storage

With a requirement for only room temperature storage, this kit maintains reagents' stability and decreases logistical challenges. Packaged for 50 preparations, it's well-suited for routine use in research, clinical, or industrial labs across India.

FAQ's of Microbiome DNA Extraction Kit:

Q: How do I use the Microbiome DNA Extraction Kit for different sample types?

A: This kit is designed for stool, soil, and swab samples. Simply follow the protocol specific to each sample type in the user manual, using the provided lysis buffer and processing the sample through the spin columns to obtain purified DNA.

Q: What advantages does the spin column format provide during extraction?

A: The spin column format ensures rapid, efficient separation and purification of DNA, reducing handling time. It also allows for easy scalability in both manual setups and automated systems.

Q: When should I use this kit in my workflow?

A: Use the kit whenever you require high-yield, quality DNA extraction from microbiome-rich samples within an hour, supporting workflows in molecular biology, clinical diagnostics, or environmental testing.

Q: Where can I safely store the kit and for how long?

A: The kit can be stored at room temperature, simplifying storage requirements. Check the product packaging or manual for specific shelf life information, but normal indoor conditions in your lab are sufficient.

Q: What is the process for extracting DNA using this kit?

A: Samples are first lysed using the included buffer, then loaded onto spin columns. Following a series of washes, high-purity DNA is eluted in 50-100 l, ready for use in downstream applications.

Q: Can this kit be used in automated extraction systems?

A: Yes, the kit is compatible with both manual and automated platforms, offering flexibility based on your lab resources and throughput needs.

Q: What are the primary benefits of using this kit over traditional DNA extraction methods?

A: Compared to conventional protocols, this kit delivers faster extraction, higher DNA yield, reliable performance across different sample types, and reduced risk of contamination due to its closed-column design.

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