Feces RNA Extraction Kit

Feces RNA Extraction Kit Specification

Extraction time
~1 hour

Format
Ready-to-use kit

Automation compatible
Yes

Total yield
Up to 100 µg

Detection method
Column-based

Includes manual
Yes

Column capacity
800 µl

Sample input
Stool/feces

RNase-free
Yes

Sample size range
200mg - 500mg

About Feces RNA Extraction Kit

Key features and details
The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.
Product Description
The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the extracted DNA. The SPINeasy RNA Kit for Feces integrates our 1) state of art bead beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNAse I treatment allows the complete removal of DNA within 1-2 minutes, further reducing the processing time.

The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

Performance

The SPINeasy RNA Kit for Feces has been developed to handle a wide variety of feces samples. When tested on various samples, the SPINeasy RNA Kit for Feces delivered higher yield, optimal 260/280 and 260/230 ratio and similar or better integrity than competitor Q kit indicating a high extraction performance across a wide range of samples (Figure 1).

Unlike competitor Q kit, no cross-DNA contamination was observed in RNA samples extracted with the SPINeasy RNA Kit for Feces. As shown in Agilent virtual gel (Figure 1, red rectangle) or agarose gel profile (Figure 2), some RNA samples extracted with the competitor Q kit contained DNA contamination; this highlights the outstanding performance of the SPINeasy RNA Kit for Feces as compared to other similar commercial kit on the market.

Application Notes
The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.
Usage Statement
MP Biomedicals
Key Applications
Genomic, Molecular Biology, PCR, qPCR

Rapid and Efficient RNA Extraction

This kit streamlines RNA isolation from fecal specimens, delivering results in approximately one hour. The intuitive column-based workflow reduces technical complexity, allowing for quick processing while maintaining sample quality. Suitable for routine laboratory tasks and larger-scale studies alike.

Designed for Flexibility and Automation

With a broad sample size range and automation compatibility, this kit accommodates diverse research needs. The columns can process up to 800 l at a time, ensuring efficiency for varying sample loads. The kit's format and included manual make it ideal for both experienced users and newcomers.

FAQ's of Feces RNA Extraction Kit:

Q: How does the Feces RNA Extraction Kit ensure RNA purity and integrity?

A: The kit utilizes a column-based purification method and all reagents provided are RNase-free, reducing the risk of RNA degradation and guaranteeing high-quality yields suitable for sensitive downstream applications.

Q: What sample sizes can be processed with this kit, and how much RNA can I expect to obtain?

A: You can process stool or feces samples ranging from 200mg to 500mg per extraction. The total RNA yield can reach up to 100 g, depending on the sample input and integrity.

Q: When should I consider using this kit over other extraction methods?

A: Choose this kit when you require a rapid, reliable protocol for fecal RNA extraction within about one hour. It's especially beneficial when working with multiple samples or seeking an automatable workflow.

Q: Where is this Feces RNA Extraction Kit available?

A: This kit is manufactured, supplied, and traded in India, making it readily accessible to laboratories across the country.

Q: What is the process for extracting RNA using this kit?

A: The kit includes a step-by-step manual guiding you through sample preparation, lysis, and column-based purification, culminating in the elution of pure RNA in under an hour.

Q: Is the kit suitable for use in automated lab equipment?

A: Yes, the kit is designed to be compatible with automation systems, which enhances efficiency and throughput for higher-volume applications.

Q: What are the principal benefits of using this ready-to-use kit?

A: The main advantages include time savings, consistent RNA yield and quality, reduced risk of contamination, ease of use, and versatile throughput options suitable for both small and large-scale research projects.

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