Feces RNA Extraction Kit

About Feces RNA Extraction Kit

• Key features and details

  The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

• Product Description

  The composition of fecal samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. Those compounds impair the sample homogenization, decrease both the quantity and quality of the extracted DNA. The SPINeasy RNA Kit for Feces integrates our 1) state of art bead beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNAse I treatment allows the complete removal of DNA within 1-2 minutes, further reducing the processing time.

  The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

  Performance

   The SPINeasy RNA Kit for Feces has been developed to handle a wide variety of feces samples. When tested on various samples, the SPINeasy RNA Kit for Feces delivered higher yield, optimal 260/280 and 260/230 ratio and similar or better integrity than competitor Q kit indicating a high extraction performance across a wide range of samples (Figure 1).

   

   Unlike competitor Q kit, no cross-DNA contamination was observed in RNA samples extracted with the SPINeasy RNA Kit for Feces. As shown in Agilent virtual gel (Figure 1, red rectangle) or agarose gel profile (Figure 2), some RNA samples extracted with the competitor Q kit contained DNA contamination; this highlights the outstanding performance of the SPINeasy RNA Kit for Feces as compared to other similar commercial kit on the market.

   

  Application Notes

  The SPINeasy RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

  Usage Statement

  MP Biomedicals

  Key Applications

  Genomic, Molecular Biology, PCR, qPCR