SPINeasy RNA Kit for Bacteria (with Lysing Matrix)

SPINeasy RNA Kit for Bacteria (with Lysing Matrix) Specification

CAS No
116541050

Binding Buffer included
Yes

Elution Volume
50100 l

Automation compatible
Yes

Includes Lysing Matrix
Yes

Time per prep
~30 minutes

Format
Spin column

Sample Capacity
15 ml

Bacterial Species
Gram +/-

RNA Yield
High

About SPINeasy RNA Kit for Bacteria (with Lysing Matrix)

SPINeasy RNA Kit for Bacteria is a silica-membrane spin-column kit that enables quick and convenient purification of total RNA from gram-positive and gram-negative bacteria. Included in the kit is our specially formulated RNASS solution that stabilizes and protects RNA in bacteria samples. The use of Lysis Buffer R and Lysing Matrix B in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of bacterial samples within seconds. With a simple workflow, the kit allows multiple samples to be processed simultaneously, without the use of toxic substances such as phenol and chloroform. Total RNA of high quality and integrity can be purified typically within 40 minutes and is immediately available for RT-PCR and other downstream applications.

Efficient RNA Extraction for Diverse Bacterial Samples

With the SPINeasy RNA Kit for Bacteria, users can extract RNA from both Gram-positive and Gram-negative organisms using sample volumes up to 5 ml. The included lysing matrix ensures robust cell disruption, and the spin column format delivers consistently high RNA yield.

Enhanced Automation for Streamlined Workflow

The kit's workflow is compatible with automation platforms, simplifying high-throughput RNA extraction. Researchers can complete each preparation in roughly 30 minutes, enabling timely downstream analysis.

FAQ's of SPINeasy RNA Kit for Bacteria (with Lysing Matrix):

Q: How does the SPINeasy RNA Kit for Bacteria ensure efficient cell lysis for both Gram-positive and Gram-negative species?

A: The kit includes a specialized lysing matrix that physically disrupts bacterial cell walls, ensuring efficient lysis of both Gram-positive and Gram-negative bacteria. This guarantees high RNA recovery regardless of the bacterial type.

Q: What is the typical process for using the SPINeasy RNA Kit for Bacteria?

A: Using this kit involves adding the sample (1-5 ml) to the provided lysing matrix, followed by disruption, binding RNA to the spin column, washing, and eluting in 50-100 l. The kit also includes binding buffer for optimal RNA capture.

Q: When is automation recommended when utilizing the SPINeasy RNA Kit for Bacteria?

A: Automation is recommended when processing multiple samples or for high-throughput applications, as the kit is fully compatible with automated liquid handling systems, making RNA extraction both consistent and efficient.

Q: Where can researchers purchase the SPINeasy RNA Kit for Bacteria in India?

A: This kit is available through authorized manufacturers, suppliers, and traders across India. The product is associated with CAS No: 116541050 for ordering and reference.

Q: What are the main benefits of using the SPINeasy RNA Kit for Bacteria?

A: Key benefits include high RNA yield, rapid extraction time (~30 minutes), compatibility with a range of bacterial species, support for automation, and reliable results with included binding buffer and spin column format.