DNA Pro Soil Extraction Kit

DNA Pro Soil Extraction Kit Specification

Size
50 Preps

Usage
Research Use Only (RUO).

Storage Instructions
Store at Room Temperature (15-25 Degree Celsius ) in a dry environment

CAS No
116546050

About DNA Pro Soil Extraction Kit

Soil samples are complex environments characterized by the presence of inhibitory compounds, such as humic acid, heavy metals, and other aromatic components which may prove to be challenging for downstream analyses. The SPINeasy DNA Pro Kit for Soil has been carefully designed for the isolation of pure microbiome genomic DNA from challenging soil types including those with low biomass or those highly contaminated. The SPINeasy DNA Pro Kit for Soil effectively lyses various microbiome population, including bacteria, fungi, viruses, and protists. The kit provides similar yields to that of our highly cited FastDNA SPIN Kit, but with improved purity and reduced processing time. Isolated DNA products showed no inhibition in PCR and were immediately ready to be used for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome) without the need of further inhibitor removal step.
Performance:-

Figure 1. DNA quality. Soil sample with various biomass/ contaminant content (250 mg each) were processed with SPINeasy DNA Pro Kit for Soil and/or competitor kit. The medium biomass group include samples stored at -20C for 12-24 months which may negatively affect the yield and integrity of the isolated DNA. The DNA yield and purity (A260/280 and A260/230 ratio) were assessed using spectrophotometer in quadruplicate when the samples were within the detection range. Each dot of the plot represents a single extraction. The horizontal bars indicate the median value. The DNA integrity was assessed using DNA gel. For low biomass samples, similar proportion of DNA eluates were loaded to compare the performance of SPINeasy DNA Pro Kit for Soil and competitor kit (Q pro).

Figure 2. Amplifiability. The absence of inhibitor was assessed using inhibitor-sensitive PCR and undiluted sample as well as quantitative PCR. 16s microbial analyses. The hypervariable region V4 of the bacterial 16S rRNA gene was amplified using DNA extracted from a high biomass soil using the 4 extraction kits described in the legend. Sequences were obtained using a NovaSeq PE250 platform and analyzed using the Qiime 2 pipeline. The relative abundance of bacterial species compilated from 3 technical replicates is shown on the left. The percentage indicates the average proportion of gram positive firmicutes (yellow) found in DNA samples derived from the same soil sample. The rarefaction curves corresponding to each method are depicted on the right. The alpha diversity was measured by the number of operational taxonomic units (OTUs) identified (vertical axis) following the sequencing depth (horizontal axis).
Get Your Free Sample Today!
Contact us for your SPINeasy DNA Pro Kit for Soil (SKU: 116546000), 5 preps here

Request samples
Application Notes
The SPINeasy DNA Pro Kit for Soil quickly and effectively isolates microbial gDNA from various soil samples. This kit is ideal for soil microbiome studies from low and high biomass soils, as it provides unbiased lysis of various microbial populations, such as bacteria, fungi, viruses, and protists. Isolated DNA exhibited a lack of inhibition in PCR and was immediately ready for downstream applications, including long fragment PCR, qPCR, and next-generation sequencing (16S and whole genome), without the need of further inhibitor removal steps.
Usage Statement
Research Use Only (RUO).
Key Applications
Genomic, Molecular Biology, Nucleic Acid Isolation & Purification, PCR, qPCR, NGS, Shotgun sequencing, Microbiome analysis