Ethidium Bromide, 5gm

Ethidium Bromide, 5gm Specification

Melting Point
260-262 deg C

CAS No
1239-45-8

Grade
Lab Grade

Purity(%)
98%

About Ethidium Bromide, 5gm

Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 g/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

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