Ethidium Bromide, 5gm

Ethidium Bromide, 5gm Specification

Melting Point
260-262 deg C

CAS No
1239-45-8

Grade
Lab Grade

Purity(%)
98%

Packaging Type
Bottle

Physical State
Solid

Molecular Weight
394.32 g/mol

Purity
High

Application
DNA staining

Shelf Life
36 months

Flash Point
>250°C

Type
Intercalating agent

Usage
Molecular biology

Appearance
Reddish brown powder

Density
~1.55 g/cm³

Storage Instructions
Store in dark

Size
5 gm

Molecular Formula
C21H20BrN3

About Ethidium Bromide, 5gm

Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 g/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Efficient DNA Staining for Molecular Biology

Ethidium Bromide serves as an essential reagent for visualizing nucleic acids during electrophoresis. Its high purity and robust staining capacity make it ideal for molecular biology laboratories, offering reliable results in DNA analysis and related experiments.

Optimal Storage for Maximum Shelf Life

For best results, Ethidium Bromide should be stored in a dark, cool place, as directed. Proper storage in its original bottle prevents degradation and ensures the reagent remains effective for up to 36 months.

Lab-Grade Quality and Packaging

This product is provided in solid form, with a density of about 1.55 g/cm3, and packaged securely in a 5-gram bottle. It is tailored for laboratory professionals, traders, and researchers across India, maintaining the highest quality standards for consistent outcomes.

FAQ's of Ethidium Bromide, 5gm:

Q: How should Ethidium Bromide be safely stored for optimal longevity?

A: Ethidium Bromide should be kept in its original tightly closed bottle and stored in a dark area, away from sunlight, to preserve its stability and potency throughout its 36-month shelf life.

Q: What is the primary application of Ethidium Bromide in molecular biology?

A: Ethidium Bromide is predominantly used as a DNA staining agent in gel electrophoresis, where it intercalates with nucleic acids, allowing for the visualization of DNA under ultraviolet light.

Q: When should Ethidium Bromide be added during the electrophoresis process?

A: Typically, Ethidium Bromide is mixed with the agarose gel either during gel preparation or post electrophoresis via staining, depending on laboratory protocols and desired sensitivity.

Q: Where is Ethidium Bromide manufactured and supplied?

A: This high-purity, lab-grade Ethidium Bromide is manufactured, supplied, and traded by reputable sources in India, and is distributed nationwide for laboratory use.

Q: What benefits does using Ethidium Bromide provide in DNA research?

A: Ethidium Bromide offers high sensitivity and rapid detection of DNA fragments, enabling efficient and accurate results in molecular biology studies and routine laboratory analyses.

Q: Is Ethidium Bromide safe to handle, and what precautions should be considered?

A: Ethidium Bromide should be handled with care, using proper protective equipment such as gloves and goggles, due to its mutagenic properties. Dispose of it according to your laboratory's hazardous waste protocols.

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