RNA Blood RNA Extraction Kit

About RNA Blood RNA Extraction Kit

• Key features and details
  • Superior yield, purity, and integrity.
  • Fast DNase treatment time (as short as 3 min).
  • Total processing time in less than 15 min.
  • Simplified buffer systems with 1 lysis buffer and 2 wash buffers
  • Product Description

    The SPINeasy RNA Kit for Blood is an advanced RNA extraction solution leveraging state-of-the-art silica-membrane spin-column technology. Specifically designed for the isolation of RNA from whole blood preserved in various anticoagulants, this kit streamlines the process to under 15 minutes. RNA preservation is paramount, and the Lysis Buffer SL1 eliminates the need for excessive homogenization while ensuring immediate RNase inactivation, preventing RNA degradation.

    Notably, the SPINeasy RNA Kit for Blood eliminates the requirement for prior erythrocyte lysis and leukocyte centrifugation, simplifying the RNA purification process. The inclusion of Fast DNase facilitates efficient on-column DNA digestion, swiftly removing co-purified DNA bound to the silica membrane. Dual washing steps have been optimized to effectively eliminate salts, metabolites, and large cellular components, contributing to a hassle-free and highly time-efficient RNA purification. Following these steps, the elution of high-yield and pure RNA is achieved using RNase-free water. The resulting RNA is well-suited for various downstream applications, including PCR, RT-qPCR amplification, and sequencing.

    Performance

    Figure 1: SPINeasy RNA Kit for Blood provided higher RNA yield, purity and comparable or higher RNA integrity in comparison to the competitor kits. Frozen whole blood samples (200 L) were processed using the SPINeasy RNA Kit for Blood and compared with the Competitor M, Z and N kits. Yields were measured with spectrophotometric and fluorometric quantification. Each dot on the plot represents a single extraction.

    Figure 2: SPINeasy RNA Kit for Blood delivered the highest yield of amplifiable extracted RNA samples. RNA samples extracted from SPINeasy RNA Kit for Blood and the various competitors were amplified in 15 replicates each for the human GAPDH gene (106bp).

    Application Notes

    The SPINeasy RNA Kit for Blood was designed for rapidly isolating high-quality RNA from whole blood samples.

    Usage Statement

    Research Use Only (RUO)

    Key Applications

    qPCR amplification

Fast and Reliable Extraction

Biogenix RNA Blood RNA Extraction Kit enables extraction of pure RNA in under 20 minutes, significantly reducing laboratory processing time. With pre-formulated reagents and included DNase, the kit consistently delivers high yields. This streamlined approach benefits researchers seeking efficiency without sacrificing result integrity.


Versatile Sample Handling

Suitable for sample volumes ranging from 50 to 300 L, this kit accommodates both small and moderate input amounts. Its versatility makes it ideal for various protocols and research needs. Additionally, the compact format reduces reagent waste and simplifies workflow for busy labs.


Certified Quality and User Assurance

Every Biogenix kit includes a Safety Data Sheet and a user-friendly instruction manual, ensuring safe handling and accurate operation. Quality certification guarantees reproducibility across batches, making it a trusted solution for blood RNA extraction needs in India.