Kanamycin Monosulfate, 735 MCG/MG

Kanamycin Monosulfate, 735 MCG/MG Specification

Molecular Formula
C18H36N4O11-H2SO4

CAS No
25389-94-0

Usage
Protein synthesis inhibitor | Selective agent

Physical State
powder

About Kanamycin Monosulfate, 735 MCG/MG

Kanamycin Monosulfate used in biotechnology applications to inhibit protein synthesis. It is also used as a selection agent for cells transformed with kanamycin B (neoR, kanR) resistance gene.

Potent Protein Synthesis Inhibition

Kanamycin Monosulfate, by disrupting prokaryotic ribosomal function, serves as a highly effective protein synthesis inhibitor. This mode of action makes it a crucial tool for preventing unwanted bacterial growth during experiments in genetic engineering, cloning, or microbiological research.

Primary Uses in Laboratory Settings

As a selective agent, Kanamycin Monosulfate is commonly used to facilitate the growth of only genetically modified organisms containing kanamycin resistance genes. Its dependable specificity ensures clarity in selection, supporting robust experimental workflows in both academic and industrial settings.

Reliable Supply Across India

Manufactured, supplied, and traded in India, Kanamycin Monosulfate is readily available for institutions and research facilities nationwide. Its powder form offers extended shelf life and simplified handling, meeting the needs of modern laboratories for quality and convenience.

FAQ's of Kanamycin Monosulfate, 735 MCG/MG:

Q: How is Kanamycin Monosulfate utilized as a selective agent in the laboratory?

A: Kanamycin Monosulfate is added to growth media to inhibit the proliferation of bacteria that are not resistant to it. This allows only genetically modified organisms with kanamycin resistance to survive and grow, making it invaluable for selecting recombinant cells during cloning experiments.

Q: What are the main benefits of using Kanamycin Monosulfate in research applications?

A: The primary advantages include its high efficacy in preventing contamination from sensitive bacteria, reliable performance in genetic selection assays, and compatibility with a variety of molecular biology protocols requiring precise control over microbial populations.

Q: When should Kanamycin Monosulfate be introduced into the culture medium?

A: It is typically added to the sterile medium after autoclaving and cooling to an appropriate temperature to avoid degradation. This ensures the compound retains its potency and selective function throughout the experimental process.

Q: Where is Kanamycin Monosulfate manufactured and can it be sourced locally in India?

A: This product is both manufactured and supplied by Indian companies, making it widely accessible to research and industrial laboratories within the country.

Q: What is the general process of using Kanamycin Monosulfate in molecular biology experiments?

A: Researchers dissolve the powdered Kanamycin Monosulfate in sterile water to prepare a stock solution. This stock is then diluted to the desired concentration in media, ensuring uniform distribution for effective selection of resistant cells.

Q: How does Kanamycin Monosulfate function as a protein synthesis inhibitor?

A: Kanamycin binds to the 30S subunit of prokaryotic ribosomes, disrupting the formation of functional proteins and thereby inhibiting bacterial growth. This action underpins its use as both an antibiotic and a selective agent in molecular biology.

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