Deoxyribonuclease I, 20mg

Deoxyribonuclease I, 20mg Specification

Handling
Avoid repeated freeze/thaw

Solubility
Water soluble

Endotoxin Level
Low

Shipping
On dry ice

Lot Tested
Yes

Activity
>2000 U/mg

pH Range
7.0-8.0

About Deoxyribonuclease I, 20mg

DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from MP Biomedicals has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas.

Exceptional Enzyme Purity and Performance

Deoxyribonuclease I, 20mg, stands out for its high enzymatic activity (>2000 U/mg) and low endotoxin content, making it reliable for sensitive biological applications. Its water solubility and stable performance in pH 7.0-8.0 enable ease of use in various laboratory protocols. Lot testing ensures batch-to-batch consistency, instilling confidence in reproducibility and experimental results.

Optimal Storage and Shipping Solutions

To maximize the enzyme's stability, Deoxyribonuclease I is shipped on dry ice. Proper handling, including avoidance of repeated freeze/thaw cycles, preserves the enzyme's activity. The recommended storage conditions and packaging protect the product during transportation across India, guaranteeing delivery in its optimal state for immediate use.

FAQ's of Deoxyribonuclease I, 20mg:

Q: How should Deoxyribonuclease I be stored to maintain its activity?

A: Deoxyribonuclease I should be stored at low temperatures, avoiding repeated freeze/thaw cycles. It is shipped on dry ice to preserve its activity, and should be kept in a suitable freezer upon arrival.

Q: What are the primary benefits of using this Deoxyribonuclease I in research applications?

A: This enzyme offers high activity (>2000 U/mg) and low endotoxin levels, making it ideal for sensitive molecular biology experiments and cell culture applications where purity and reliability are essential.

Q: When is it best to use Deoxyribonuclease I during laboratory workflows?

A: Deoxyribonuclease I is most effective when added during DNA digestion steps, such as removing contaminating DNA from protein preparations or processing cell lysates in molecular biology and diagnostics.

Q: Where is Deoxyribonuclease I supplied from and how is it delivered?

A: It is provided by a manufacturer, supplier, and trader based in India and is shipped on dry ice to ensure stability throughout transit.

Q: What is the recommended pH range for optimal Deoxyribonuclease I performance?

A: The enzyme demonstrates optimal activity in the pH range of 7.0 to 8.0, which aligns with most physiological and experimental conditions.

Q: How is product consistency ensured for this batch of Deoxyribonuclease I?

A: Every lot is tested to verify performance and quality, ensuring that each batch meets rigorous specifications for enzymatic activity and purity before being released.

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