Tris-Borate-Edta Buffer

Tris-Borate-Edta Buffer Specification

Usage
Laboratory

Storage Instructions
Room Temperature

CAS No
04816721

Grade
Reagent Grade

Molecular Formula
C6H15NO3·B(OH)3

Ph Level
8.3

Shelf Life
24 months

Appearance
Clear liquid

Physical State
Liquid

Size
500 ml

Packaging Type
HDPE bottle

Purity
High

Molecular Weight
Varies

Application
Molecular biology

Purity(%)
99%

Type
Buffer solution

About Tris-Borate-Edta Buffer

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Applications in Molecular Biology

TBE Buffer is pivotal for laboratories engaged in gene analysis, DNA/RNA electrophoresis, and nucleic acid separation. Its stable formulation supports consistent pH and conductivity, critical for precise molecular biology workflows.

Storage and Packaging

Packaged securely in an HDPE bottle, this buffer solution ensures safety and easy dispensing. With a shelf life of up to 24 months, it remains reliable when stored at room temperature, making it convenient for most laboratory environments.

Quality and Purity Assurance

With a remarkable 99% purity and reagent-grade classification, the buffer guarantees minimal contaminants, which translates to accuracy in laboratory results. Each batch undergoes stringent quality checks to match international standards.

FAQ's of Tris-Borate-Edta Buffer:

Q: How should Tris-Borate-Edta Buffer be stored for optimal stability?

A: TBE Buffer should be stored at room temperature in its original HDPE bottle, away from direct sunlight and extreme heat to maintain its effectiveness throughout the 24-month shelf life.

Q: What are the main uses of TBE Buffer in molecular biology laboratories?

A: It is primarily used in nucleic acid electrophoresis for separating DNA and RNA, ensuring sharp band resolution and consistent results in molecular genetics research.

Q: When is it advisable to use TBE Buffer instead of other buffer solutions?

A: TBE Buffer is ideal when high electrophoretic clarity, reduced background, and stable pH are needed, particularly in DNA/RNA analyses where precise migration and resolution are essential.

Q: Where can TBE Buffer be sourced in India?

A: It is available from reputable manufacturers, suppliers, and traders across India, often stocked by scientific supply companies catering to laboratory needs.

Q: What process does one follow to prepare electrophoresis gels with TBE Buffer?

A: Typically, the buffer is diluted to the desired concentration and mixed with agarose powder. The solution is then heated and cast into a gel tray, upon which samples are run using the prepared buffer.

Q: Why is Tris-Borate-Edta Buffer preferred for laboratory work?

A: Its high purity, reagent-grade quality, and robust buffering capacity provide scientists with reliable, reproducible results, making it an essential component in precise laboratory protocols.

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