About GENECLEAN Turbo for PCR
The GENECLEAN Turbo for PCR Kit is a specially optimized kit for PCR purification of DNA fragments of sizes 100 bp to 10 kb. This kit uses the same cartridge housing as the GENECLEAN Turbo Kit. However, it contains a unique membrane optimized for PCR product purification. It purify larger fragments due to less manipulation of bound DNA. Process with either vacuum or centrifugation & save time with patented Turbo filter design.
Fast and Reliable PCR PurificationGENECLEAN Turbo for PCR stands out with its rapid processing time-purify your PCR products in only five minutes. The spin filter column format makes the process user-friendly and suitable for both new and experienced users. By ensuring minimal hands-on intervention and high recovery, the kit is an efficient choice for labs handling multiple samples daily.
High Yield and Versatile Elution VolumesThis kit supports binding of up to 10 g of DNA per column, offering strong compatibility with a range of PCR product sizes and concentrations. Users can adjust the elution volume between 6 and 50 l to achieve their desired DNA concentration, suiting different downstream requirements. High recovery yields mean more DNA for your applications from every run.
Comprehensive Contaminant RemovalGENECLEAN Turbo for PCR is specifically formulated to efficiently remove a wide array of PCR reaction contaminants, including primers, unincorporated nucleotides, residual enzymes, salts, and even mineral oil. This ensures the DNA you elute is purified and ready for sensitive downstream applications such as sequencing, cloning, or enzymatic reactions.
FAQ's of GENECLEAN Turbo for PCR:
Q: How do I use the GENECLEAN Turbo for PCR kit to purify DNA from PCR reactions?
A: Simply add your PCR sample or agarose gel fragment to the spin filter column, follow the quick protocol steps, and elute your DNA in 6-50 l. The entire process takes just five minutes and yields highly pure DNA suitable for downstream applications.
Q: What sample types and contaminants can GENECLEAN Turbo for PCR handle?
A: The kit is designed for purification of DNA from PCR reactions and agarose gel fragments. It effectively removes primers, nucleotides, enzymes, salts, and mineral oil from the final eluted product.
Q: When is it best to use GENECLEAN Turbo for PCR during my workflow?
A: Use the kit immediately after your PCR reaction or gel extraction to efficiently isolate DNA before moving on to sensitive downstream processes such as sequencing, cloning, or restriction digestion.
Q: Where should I store the GENECLEAN Turbo for PCR kit?
A: It should be stored at a controlled room temperature, specifically between 15C and 30C, to maintain reagent stability and optimal performance.
Q: What is the binding capacity and elution range of the GENECLEAN Turbo for PCR kit?
A: Each spin filter column can bind up to 10 g of DNA. The elution volume can be adjusted between 6 and 50 l, providing flexibility for different DNA concentration needs.
Q: How does using this kit benefit my DNA purification process?
A: The kit offers high recovery yields, convenience in a ready-to-use format, rapid 5-minute processing, and efficient removal of multiple contaminants, all contributing to reliable, high-purity DNA for your research.