RPMI 1640, w/o L-Glutamine

RPMI 1640, w/o L-Glutamine Specification

Purity
High

Packaging Type
Bottle

Physical State
Liquid

Shelf Life
1 Years

Size
500ml

CAS No
091261554

Type
Industrial Lab Chemicals

RPMI 1640, w/o L-Glutamine Trade Information

Minimum Order Quantity
1 Piece

Delivery Time
5-10 Days

About RPMI 1640, w/o L-Glutamine

RPMI 1640 is a growth medium used in different cell culture applications. RPMI stands for Roswell Park Memorial Institute, where it was developed in 1966. This formulation of RPMI 1640 comes without L-Glutamine. RPMI 1640 was developed to support lymphoblastoid cells in suspension cultures. It can also support a wide range of other cell types such as HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Originally intended to be supplemented with serum, RPMI 1640 has been shown to also support several cell lines in the absence of serum.

Versatile and High-Quality Cell Culture Medium

RPMI 1640 w/o L-Glutamine offers researchers and industrial labs a flexible base for customizing cell culture according to experiment needs. Its liquid state ensures even distribution and easy handling, while the absence of L-Glutamine allows direct control over glutamine supplementation, which is critical for optimizing cell growth conditions.

Reliable Packaging and Convenient Sizing

Packaged securely in a 500ml bottle, this product ensures minimal contamination risk and user convenience during transfers. The bottle format is ideal for routine laboratory practices, supporting both small and large-scale experimental workflows.

FAQ's of RPMI 1640, w/o L-Glutamine:

Q: How should RPMI 1640 w/o L-Glutamine be stored to maintain its high purity and effectiveness?

A: RPMI 1640 w/o L-Glutamine should be stored at 2-8C, away from direct light, to preserve its purity and prevent degradation. Ensure the bottle is tightly sealed when not in use to maintain the product's stability throughout its 1-year shelf life.

Q: What are the primary uses of RPMI 1640 medium without L-Glutamine in industrial laboratories?

A: This medium is primarily used for culturing mammalian cells, particularly lymphocytes and hybridomas, in research and industrial applications. The absence of L-Glutamine allows researchers to add it as needed, optimizing conditions for specific cell types.

Q: Where can I purchase RPMI 1640 w/o L-Glutamine in India?

A: The product is available through multiple manufacturers, suppliers, and traders across India. It is typically supplied in 500ml bottles, ensuring you receive it in secure and standardized packaging suitable for industrial lab use.

Q: When should I add L-Glutamine to RPMI 1640 w/o L-Glutamine?

A: L-Glutamine should be added immediately before use or as recommended for your specific cell culture protocol. This custom addition allows more precise control over cell growth environments, reducing possible decomposition of L-Glutamine during storage.

Q: How is RPMI 1640 w/o L-Glutamine supplied and what are the packaging benefits?

A: It is supplied as a ready-to-use liquid in a 500ml bottle, providing high convenience for laboratory workflows. The bottle packaging reduces contamination risk and allows for easy measurement and transfer during experiments.

Q: What are the benefits of using RPMI 1640 without L-Glutamine for cell culture?

A: Using this medium grants researchers flexibility to optimize glutamine concentration, extend medium stability, and maintain high control over experimental variables, resulting in more reproducible and reliable cell culture outcomes.

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