5-Bromo-4-Chloro- 3-Indolyl-Beta -D-Galactopyranoside, 100mg

5-Bromo-4-Chloro- 3-Indolyl-Beta -D-Galactopyranoside, 100mg Specification

Storage
Keep away from moisture

Purity
High

CAS No
7240-90-6

Grade
Industrial Grade

Usage
Laboratory

5-Bromo-4-Chloro- 3-Indolyl-Beta -D-Galactopyranoside, 100mg Trade Information

Minimum Order Quantity
1 Kilograms

Supply Ability
1000 Kilograms Per Month

Delivery Time
7 Days

About 5-Bromo-4-Chloro- 3-Indolyl-Beta -D-Galactopyranoside, 100mg

5-Bromo-4-Chloro-3-Indolyl--D-Galactopyranoside is used as indigogenic substrate for -galactosidase, for detection of -galactosidase-positive clones, and the identification of lac and bacterial colonies or phage plaques. It is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype. XGal is cleaved by galactosidase to yield an insoluble blue precipitate. XGal is particularly useful in molecular biology applications to detect the activity of galactosidase which is frequently used as a reporter gene. In cloning, XGal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using -complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the -galactosidase gene encoding an -fragment of -galactosidase.

High-Purity Reagent for Reliable Results

Engineered for laboratory use, this X-Gal compound offers high purity, ensuring dependable results in molecular biology assays. The industrial-grade quality makes it suitable for research and experimental processes where accuracy is essential. Trusted by manufacturers, suppliers, and traders across India, it helps facilitate consistent screening outcomes.

Optimal Storage to Preserve Effectiveness

This reagent should always be kept away from moisture to maintain its integrity and extend shelf life. Proper storage ensures that 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside delivers precise and consistent results throughout its usage. Following correct procedures will help laboratories maximize the utility of each 100mg batch.

FAQ's of 5-Bromo-4-Chloro- 3-Indolyl-Beta -D-Galactopyranoside, 100mg:

Q: How should 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside be stored for optimal results?

A: This reagent should be kept away from moisture to preserve its stability and effectiveness. Store it in a tightly sealed container at a cool, dry place to prevent degradation.

Q: What are the primary applications of 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside in laboratory settings?

A: This compound is mainly used for blue-white screening in molecular biology. It helps in the detection of beta-galactosidase activity, allowing researchers to distinguish between recombinant and non-recombinant bacterial colonies.

Q: When is it recommended to use industrial-grade X-Gal in experiments?

A: Industrial-grade X-Gal is suitable for research and experimental procedures when you require high purity and consistent performance, particularly in molecular genetics and microbiology.

Q: Where can laboratories in India procure this product?

A: 5-Bromo-4-Chloro-3-Indolyl-Beta-D-Galactopyranoside is available through various manufacturers, suppliers, and traders in India specializing in laboratory chemicals and reagents.

Q: What is the process of using X-Gal for blue-white screening?

A: X-Gal is incorporated into agar plates along with bacterial cultures and appropriate selection markers. When beta-galactosidase is present, bacterial colonies turn blue, facilitating straightforward screening.

Q: Can you highlight the key benefits of using this high-purity reagent?

A: The main advantages include reliable experimental outcomes, clear differentiation in screening assays, and suitability for sensitive molecular biology techniques, all aided by its high purity and stable formulation.

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