SPINeasy PCR Purification and Gel Extraction Kit

SPINeasy PCR Purification and Gel Extraction Kit Specification

Storage Instructions
Store at Room Temperature (15-25 Degree Celsius), Dry environment

Column Number
50 columns/kit

Processing Time
<30 minutes

Binding Capacity
Up to 10 µg DNA

DNA Fragment Size
70 bp-10 kb

Compatibility
Agarose gel DNA

Minimum Elution Volume
15 µl

Kit Format
Spin column-based

Target
dsDNA recovery

Sample Size
Up to 100 mg gel

Application
PCR purification, gel extraction

About SPINeasy PCR Purification and Gel Extraction Kit

Key features and details
Enable quick and convenient DNA clean-up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis, using silica-membrane spin column

Reliable and Rapid DNA Purification

Achieve high-quality dsDNA recovery quickly and effortlessly with the SPINeasy kit's efficient spin column technology. Process up to 50 samples per kit, with completion times typically less than 30 minutes, making it suitable for demanding laboratory workflows. The streamlined protocol ensures consistent results for both PCR purification and agarose gel extraction applications.

Versatility Across Applications

The SPINeasy kit is compatible with a range of downstream processes, enabling the purification of DNA fragments from 70 bp to 10 kb. Whether your workflow involves PCR purification or extraction from agarose gels, this kit delivers robust performance and flexibility, adapting seamlessly to various molecular biology projects.

Convenient Storage and Robust Performance

Designed for easy storage at room temperature (15-25C), the SPINeasy kit maintains reliability and performance in dry environments. This convenience minimizes the need for cold storage, making the kit well-suited to a variety of laboratory settings in India and beyond.

FAQ's of SPINeasy PCR Purification and Gel Extraction Kit:

Q: How do I use the SPINeasy PCR Purification and Gel Extraction Kit for DNA recovery?

A: Simply add your PCR product or gel-dissolved DNA sample to the provided spin column, follow the included protocol for binding, washing, and elution, and retrieve purified double-stranded DNA in as little as 15 l elution volume.

Q: What types of DNA fragments and samples can this kit process?

A: The kit efficiently purifies double-stranded DNA fragments ranging from 70 bp to 10 kb from PCR mixtures or agarose gel slices, accommodating up to 100 mg of gel per spin column.

Q: When should I choose this kit for my laboratory workflow?

A: This kit is ideal whenever you require rapid purification of high-yield dsDNA from PCR reactions or for extracting DNA from agarose gels. It is especially suitable for workflows that demand quick turnaround and high reproducibility.

Q: Where can I store the SPINeasy kit and what are the storage requirements?

A: You should store the kit at room temperature, specifically between 15 and 25C, in a dry environment to maintain optimal reagent integrity and shelf life.

Q: What is the process time for purifying DNA using this kit?

A: The entire purification or extraction process can be completed in under 30 minutes, from sample preparation to DNA elution, enabling efficient use of your lab time.

Q: Is the SPINeasy kit compatible with agarose gel DNA extractions?

A: Yes, it is fully compatible. The kit enables recovery of DNA directly from agarose gel fragments, providing flexibility for various molecular biology protocols.

Q: What benefits does this kit offer compared to traditional purification methods?

A: The SPINeasy kit offers rapid, high-purity DNA recovery with minimal hands-on time. Its spin column format ensures ease of use, high binding capacity (up to 10 g DNA), and consistent, reliable results for PCR purification and gel extraction.

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