LymphoSep lymphocyte separation medium, 100mL, 500mL

LymphoSep lymphocyte separation medium, 100mL, 500mL Specification

CAS No
0916922-CF

Size
1x 100mL & 1x 500mL

Physical State
Liquid

Type
Other

Grade
Cell culture grade

Density
1.077 g/mL

Application
Lymphocyte separation

Packaging Type
Bottle

Shelf Life
24 months

Purity
>99%

Appearance
Clear solution

Storage Instructions
Room temperature

About LymphoSep lymphocyte separation medium, 100mL, 500mL

Lymphosep - Lymphocyte Separation Medium is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinized or defibrinated human blood. Lymphocytes are isolated by layering diluted, heparinized blood over a solution of sodium diatrizoate that contains a polysaccharide. This polysaccharide aggregates the erythrocytes so that they can be sedimented by low centrifugation. The less dense lymphocytes remain as a clearly defined band at the plasma Lymphosep interface.

Maximum yield of mononuclear cells.
Easy one-step centrifugation.
>96% cell viability of lymphocytes.
Low endotoxins.
Autoclave sterilization.

Efficient Lymphocyte Isolation

LymphoSep separation medium enables the reliable and rapid isolation of viable lymphocytes from blood samples. With its high purity and cell culture compatibility, researchers can obtain high-quality cell populations for downstream applications such as immunological assays, diagnostics, and cell culture studies.

Convenient Storage and Long Shelf Life

This medium is ready-to-use and stable at room temperature, eliminating the need for cold storage. Each bottle has a shelf life of 24 months, making it economical and practical for laboratories with varying use frequencies.

High Purity for Reliable Results

Crafted to exceed 99% purity standards, LymphoSep supports precise and reproducible separation of lymphocytes. Its transparent formulation ensures optimal performance in cell culture and research settings.

FAQ's of LymphoSep lymphocyte separation medium, 100mL, 500mL:

Q: How is LymphoSep lymphocyte separation medium used in the laboratory?

A: LymphoSep is layered beneath blood samples in a centrifuge tube. Upon centrifugation, it creates a density gradient, allowing lymphocytes to be isolated at the interface between plasma and the medium.

Q: What are the main benefits of using LymphoSep for lymphocyte isolation?

A: The main benefits include its high purity (>99%), consistent cell culture grade quality, convenience of room temperature storage, and its ability to provide efficient, reproducible lymphocyte separation for research and clinical applications.

Q: When should I use LymphoSep medium in my research process?

A: Use LymphoSep when you require a concentrated, pure population of lymphocytes from whole blood or mixed cell suspensions, especially before conducting immunological assays or in vitro studies.

Q: Where can I purchase LymphoSep lymphocyte separation medium in India?

A: LymphoSep is available from reputable manufacturers, suppliers, and traders across India. It is typically offered in 100mL and 500mL bottle packaging for laboratory use.

Q: What steps are involved in the lymphocyte separation process with this product?

A: The process involves carefully layering your blood sample onto LymphoSep, centrifuging to form distinct cell layers, and then collecting the lymphocyte-rich layer formed at the interface for further use.

Q: Is any special storage required for LymphoSep medium?

A: LymphoSep should be stored at room temperature in its original bottle, tightly sealed, away from direct sunlight to preserve its integrity for the full 24-month shelf life.

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